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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Pseudomonas putida (strain W619)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Enzyme and pathway databases

BioCyciPPUT390235:G1GBA-4196-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:PputW619_4084
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000720 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001147581 – 900Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST900

Interactioni

Protein-protein interaction databases

STRINGi390235.PputW619_4084

Structurei

3D structure databases

ProteinModelPortaliB1JBR2
SMRiB1JBR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini461 – 583HDPROSITE-ProRule annotationAdd BLAST123
Domaini706 – 784ACT 1UniRule annotationAdd BLAST79
Domaini816 – 900ACT 2UniRule annotationAdd BLAST85

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 342UridylyltransferaseAdd BLAST342
Regioni343 – 705Uridylyl-removingAdd BLAST363

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF
OrthoDBiPOG091H04HO

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 1 hit
PfamiView protein in Pfam
PF01842 ACT, 1 hit
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

B1JBR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAGEVLD RRFRDGRDIR
60 70 80 90 100
RLIEDRAWLV DNILQQAWKQ FDWGDADGIA LVAVGGYGRG ELHPHSDIDL
110 120 130 140 150
LILLRDAEHE QYRDAIERFL TLLWDIGLEV GQSVRTVDEC AEQARADLTV
160 170 180 190 200
ITNMMESRTI AGREALRQRM LEVTSTAHMW PSKEFFLAKR AELKARHHKY
210 220 230 240 250
NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL AGEGFLLESE
260 270 280 290 300
NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIAAL LGYSDENPKR
310 320 330 340 350
AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADDDSGTT QPLNARFRLH
360 370 380 390 400
DGYIEAVNAN VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI
410 420 430 440 450
DDTFRNDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ
460 470 480 490 500
MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV SEKFPLASKL MGRLPKPELI
510 520 530 540 550
YLAGLYHDIG KGRQGDHSEL GAVDAKKFCE RHQLPAWDSR LIVWLVQNHL
560 570 580 590 600
VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN
610 620 630 640 650
SWRASLLRQL YSETKRALRR GLENPLDREE QIRQTQSAAL DILVREGTDP
660 670 680 690 700
DDVEQLWSQL GDDYFLKHNA ADVAWHSDAI LQQPADGGPL VLIKETTQRE
710 720 730 740 750
FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS SSQFTLDTYI
760 770 780 790 800
VLDNDGGSIG DNPQRVKQIR DGLTEALRNP EDYPTIIQRR VPRQLKHFNF
810 820 830 840 850
PPQVTILNDA QRAVTILEIT APDRPGLLAR IGRIFLEFDL SLQNAKIATL
860 870 880 890 900
GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQA SDASPTRVTF
Length:900
Mass (Da):102,914
Last modified:April 29, 2008 - v1
Checksum:iE6F3451AFEC02E74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA Translation: ACA74564.1
RefSeqiWP_012315901.1, NC_010501.1

Genome annotation databases

EnsemblBacteriaiACA74564; ACA74564; PputW619_4084
KEGGippw:PputW619_4084

Similar proteinsi

Entry informationi

Entry nameiGLND_PSEPW
AccessioniPrimary (citable) accession number: B1JBR2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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