B1JBR2 (GLND_PSEPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Ordered Locus Names:	PputW619_4084

Organism

Pseudomonas putida (strain W619) [Complete proteome] [HAMAP]

Taxonomic identifier

390235 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein By similarity. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Molecular_function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_1000114758

Sequences

	Sequence		Length	Mass (Da)
	B1JBR2 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: E6F3451AFEC02E74	FASTA	900	102,914
	10 20 30 40 50 60 MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAGEVLD RRFRDGRDIR RLIEDRAWLV 70 80 90 100 110 120 DNILQQAWKQ FDWGDADGIA LVAVGGYGRG ELHPHSDIDL LILLRDAEHE QYRDAIERFL 130 140 150 160 170 180 TLLWDIGLEV GQSVRTVDEC AEQARADLTV ITNMMESRTI AGREALRQRM LEVTSTAHMW 190 200 210 220 230 240 PSKEFFLAKR AELKARHHKY NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL 250 260 270 280 290 300 AGEGFLLESE NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIAAL LGYSDENPKR 310 320 330 340 350 360 AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADDDSGTT QPLNARFRLH DGYIEAVNAN 370 380 390 400 410 420 VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI DDTFRNDIRN TSLFIELFKC 430 440 450 460 470 480 EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV 490 500 510 520 530 540 SEKFPLASKL MGRLPKPELI YLAGLYHDIG KGRQGDHSEL GAVDAKKFCE RHQLPAWDSR 550 560 570 580 590 600 LIVWLVQNHL VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 610 620 630 640 650 660 SWRASLLRQL YSETKRALRR GLENPLDREE QIRQTQSAAL DILVREGTDP DDVEQLWSQL 670 680 690 700 710 720 GDDYFLKHNA ADVAWHSDAI LQQPADGGPL VLIKETTQRE FEGGTQIFIY APDQHDFFAV 730 740 750 760 770 780 TVAAMSQLNL NIHDARIITS SSQFTLDTYI VLDNDGGSIG DNPQRVKQIR DGLTEALRNP 790 800 810 820 830 840 EDYPTIIQRR VPRQLKHFNF PPQVTILNDA QRAVTILEIT APDRPGLLAR IGRIFLEFDL 850 860 870 880 890 900 SLQNAKIATL GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQA SDASPTRVTF

References

[1]

"Complete sequence of Pseudomonas putida W619." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Taghavi S., Vangronsveld D., van der Lelie D., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: W619.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000949 Genomic DNA. Translation: ACA74564.1.
RefSeq	YP_001750933.1. NC_010501.1.
3D structure databases
ProteinModelPortal	B1JBR2.
ModBase	Search...
Protein-protein interaction databases
STRING	390235.PputW619_4084.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA74564; ACA74564; PputW619_4084.
GeneID	6113053.
KEGG	ppw:PputW619_4084.
PATRIC	19958059. VBIPsePut93764_4107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2844.
HOGENOM	HOG000261778.
KO	K00990.
OMA	SPKVWNA.
ProtClustDB	PRK00275.
Enzyme and pathway databases
BioCyc	PPUT390235:GHHJ-4146-MONOMER.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 1 hit. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_PSEPW

Accession

Primary (citable) accession number: B1JBR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program