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B1JBR2

- GLND_PSEPW

UniProt

B1JBR2 - GLND_PSEPW

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, PputW619_4084
Organism
Pseudomonas putida (strain W619)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-4146-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PputW619_4084
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000720: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000114758Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390235.PputW619_4084.

Structurei

3D structure databases

ProteinModelPortaliB1JBR2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini481 – 590110HD
Add
BLAST
Domaini706 – 78479ACT 1
Add
BLAST
Domaini816 – 90085ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotation
Add
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1JBR2-1 [UniParc]FASTAAdd to Basket

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MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRQAGEVLD RRFRDGRDIR    50
RLIEDRAWLV DNILQQAWKQ FDWGDADGIA LVAVGGYGRG ELHPHSDIDL 100
LILLRDAEHE QYRDAIERFL TLLWDIGLEV GQSVRTVDEC AEQARADLTV 150
ITNMMESRTI AGREALRQRM LEVTSTAHMW PSKEFFLAKR AELKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL AGEGFLLESE 250
NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIAAL LGYSDENPKR 300
AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADDDSGTT QPLNARFRLH 350
DGYIEAVNAN VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI 400
DDTFRNDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV SEKFPLASKL MGRLPKPELI 500
YLAGLYHDIG KGRQGDHSEL GAVDAKKFCE RHQLPAWDSR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 600
SWRASLLRQL YSETKRALRR GLENPLDREE QIRQTQSAAL DILVREGTDP 650
DDVEQLWSQL GDDYFLKHNA ADVAWHSDAI LQQPADGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS SSQFTLDTYI 750
VLDNDGGSIG DNPQRVKQIR DGLTEALRNP EDYPTIIQRR VPRQLKHFNF 800
PPQVTILNDA QRAVTILEIT APDRPGLLAR IGRIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQA SDASPTRVTF 900
Length:900
Mass (Da):102,914
Last modified:April 29, 2008 - v1
Checksum:iE6F3451AFEC02E74
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000949 Genomic DNA. Translation: ACA74564.1.
RefSeqiYP_001750933.1. NC_010501.1.

Genome annotation databases

EnsemblBacteriaiACA74564; ACA74564; PputW619_4084.
GeneIDi6113053.
KEGGippw:PputW619_4084.
PATRICi19958059. VBIPsePut93764_4107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000949 Genomic DNA. Translation: ACA74564.1 .
RefSeqi YP_001750933.1. NC_010501.1.

3D structure databases

ProteinModelPortali B1JBR2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 390235.PputW619_4084.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA74564 ; ACA74564 ; PputW619_4084 .
GeneIDi 6113053.
KEGGi ppw:PputW619_4084.
PATRICi 19958059. VBIPsePut93764_4107.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PPUT390235:GHHJ-4146-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W619.

Entry informationi

Entry nameiGLND_PSEPW
AccessioniPrimary (citable) accession number: B1JBR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi