Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional purine biosynthesis protein PurH

Gene

purH

Organism
Pseudomonas putida (strain W619)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation
IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. IMP cyclohydrolase activity Source: UniProtKB-HAMAP
  2. phosphoribosylaminoimidazolecarboxamide formyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transferase

Keywords - Biological processi

Purine biosynthesis

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-4686-MONOMER.
UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthesis protein PurHUniRule annotation
Including the following 2 domains:
Phosphoribosylaminoimidazolecarboxamide formyltransferaseUniRule annotation (EC:2.1.2.3UniRule annotation)
Alternative name(s):
AICAR transformylaseUniRule annotation
IMP cyclohydrolaseUniRule annotation (EC:3.5.4.10UniRule annotation)
Alternative name(s):
ATICUniRule annotation
IMP synthaseUniRule annotation
InosinicaseUniRule annotation
Gene namesi
Name:purHUniRule annotation
Ordered Locus Names:PputW619_4612
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000720 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Bifunctional purine biosynthesis protein PurHPRO_1000096084Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390235.PputW619_4612.

Structurei

3D structure databases

ProteinModelPortaliB1J5T3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The IMP cyclohydrolase activity resides in the N-terminal region.UniRule annotation

Sequence similaritiesi

Belongs to the PurH family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.

Sequencei

Sequence statusi: Complete.

B1J5T3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDQTTRLPV RRALISVSDK TGILEFAREL QQLGVEILST GGTYKLLKDN
60 70 80 90 100
GVNAVEVADY TGFAEMMDGR VKTLHPKIHG GILGRRGVDD AIMNEHGIKP
110 120 130 140 150
IDLVAVNLYP FEATIAKPGC DLPTAIENID IGGPTMVRSA AKNHKDVAIV
160 170 180 190 200
VNASDYASVL EGLKAGGLTY AQRFDLMLKA FEHTAAYDGM IANYMGTIDQ
210 220 230 240 250
AKDTLSTEAR SEFPRTFNSQ FVKAQEMRYG ENPHQSAAFY VEAKKGEASV
260 270 280 290 300
STAIQLQGKE LSFNNVADTD AALECVKSFV KPACVIVKHA NPCGVAVVPE
310 320 330 340 350
DEGGIRKAYD LAYATDTESA FGGIIAFNRE LDGETAKAIV ERQFVEVIIA
360 370 380 390 400
PKISQAARDV VAAKQNVRLL ECGEWPAERA AGWDFKRVNG GLLVQSRDNG
410 420 430 440 450
MITAEDLKIV TKRAPTEQEI HDLVFAWKVA KFVKSNAIVY AKQRQTIGVG
460 470 480 490 500
AGQMSRVNSA RIAAIKAEHA GLQVQGAVMA SDAFFPFRDG IDNAAKVGIS
510 520 530
AVIQPGGSMR DAEVIAAADE AGIAMVFTGM RHFRH
Length:535
Mass (Da):57,737
Last modified:April 29, 2008 - v1
Checksum:iED9EC9D22E1063EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA75092.1.
RefSeqiWP_012316411.1. NC_010501.1.
YP_001751461.1. NC_010501.1.

Genome annotation databases

EnsemblBacteriaiACA75092; ACA75092; PputW619_4612.
KEGGippw:PputW619_4612.
PATRICi19959145. VBIPsePut93764_4638.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA75092.1.
RefSeqiWP_012316411.1. NC_010501.1.
YP_001751461.1. NC_010501.1.

3D structure databases

ProteinModelPortaliB1J5T3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390235.PputW619_4612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA75092; ACA75092; PputW619_4612.
KEGGippw:PputW619_4612.
PATRICi19959145. VBIPsePut93764_4638.

Phylogenomic databases

eggNOGiCOG0138.
HOGENOMiHOG000230373.
KOiK00602.
OMAiPCGVAEG.
OrthoDBiEOG6QCDFF.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00133.
UPA00074; UER00135.
BioCyciPPUT390235:GHHJ-4686-MONOMER.

Family and domain databases

Gene3Di3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPiMF_00139. PurH.
InterProiIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERiPTHR11692. PTHR11692. 1 hit.
PfamiPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFiPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTiSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsiTIGR00355. purH. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W619.

Entry informationi

Entry nameiPUR9_PSEPW
AccessioniPrimary (citable) accession number: B1J5T3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.