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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

PputW619_1602

Organism
Pseudomonas putida (strain W619)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981SubstrateBy similarity
Metal bindingi99 – 991Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-1640-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:PputW619_1602
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000720 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 163163Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_1000194867Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi390235.PputW619_1602.

Structurei

3D structure databases

ProteinModelPortaliB1J596.
SMRiB1J596. Positions 3-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 794Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiVTIKCHE.
OrthoDBiEOG6Z3KKK.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

B1J596-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHYVTPDLC DAYPDLVQVL EPMFSNFGGR DSFGGQIVTI KCFEDNSRVK
60 70 80 90 100
EQVELDGKGK VLVVDGGGSL RCALLGDMLA EKAAKNGWEG LVIYGCVRDV
110 120 130 140 150
DVLIQTSVGV QALASHPMKT DKRGIGDLNV AVTFAGVTFR PGEYVYADNN
160
GVIVSPSPLE MPQ
Length:163
Mass (Da):17,539
Last modified:April 29, 2008 - v1
Checksum:iA1483A4103088F8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA72107.1.
RefSeqiWP_012313510.1. NC_010501.1.

Genome annotation databases

EnsemblBacteriaiACA72107; ACA72107; PputW619_1602.
KEGGippw:PputW619_1602.
PATRICi19953003. VBIPsePut93764_1608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA72107.1.
RefSeqiWP_012313510.1. NC_010501.1.

3D structure databases

ProteinModelPortaliB1J596.
SMRiB1J596. Positions 3-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390235.PputW619_1602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA72107; ACA72107; PputW619_1602.
KEGGippw:PputW619_1602.
PATRICi19953003. VBIPsePut93764_1608.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OMAiVTIKCHE.
OrthoDBiEOG6Z3KKK.

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-1640-MONOMER.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W619.

Entry informationi

Entry nameiRRAAH_PSEPW
AccessioniPrimary (citable) accession number: B1J596
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: April 13, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.