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Protein

Tetraacyldisaccharide 4'-kinase

Gene

lpxK

Organism
Pseudomonas putida (strain W619)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).UniRule annotation

Catalytic activityi

ATP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-4-O-phospho-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl phosphate).UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 6 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 67ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT390235:G1GBA-1553-MONOMER
UniPathwayiUPA00359; UER00482

Names & Taxonomyi

Protein namesi
Recommended name:
Tetraacyldisaccharide 4'-kinaseUniRule annotation (EC:2.7.1.130UniRule annotation)
Alternative name(s):
Lipid A 4'-kinaseUniRule annotation
Gene namesi
Name:lpxKUniRule annotation
Ordered Locus Names:PputW619_1511
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000720 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003408521 – 336Tetraacyldisaccharide 4'-kinaseAdd BLAST336

Interactioni

Protein-protein interaction databases

STRINGi390235.PputW619_1511

Structurei

3D structure databases

SMRiB1J506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxK family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHD Bacteria
COG1663 LUCA
HOGENOMiHOG000004953
KOiK00912
OMAiMDDGFQN
OrthoDBiPOG091H000L

Family and domain databases

HAMAPiMF_00409 LpxK, 1 hit
InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR003758 Tetraacyldisaccharide_4-kinase
PANTHERiPTHR42724 PTHR42724, 1 hit
PfamiView protein in Pfam
PF02606 LpxK, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00682 lpxK, 1 hit

Sequencei

Sequence statusi: Complete.

B1J506-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALADRLLAA WYAGHPALAL LRPLEALYRR VVTRKRARFL SGESASYRAP
60 70 80 90 100
VPVIVVGNIT IGGTGKTPMI LWLIEHCRRQ GLKVGVVSRG YGAEPPRLPW
110 120 130 140 150
RVEAIQSAEQ AGDEPLLIVQ RTGVPLMIDP DRSRAVQALL ASEPLDLILC
160 170 180 190 200
DDGMQHYRLA RDLELVLIDA ARGLGNGRCL PAGPLREPAE RLQEVDAVLF
210 220 230 240 250
NGASADRPEG FAFHLQPSAL VNLRTGERRA LDFFPAGQRL HAVAGIGNPQ
260 270 280 290 300
RFFNTLLGLN WQPVPHPFAD HAQFSAQSLA FSPSLPLVMT EKDAVKCRAF
310 320 330
AADDWWYLAV EALPTPAFCG WFDSQLQRLL PGHRTP
Length:336
Mass (Da):36,976
Last modified:April 29, 2008 - v1
Checksum:i11E66476BCC185A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA Translation: ACA72016.1
RefSeqiWP_012313422.1, NC_010501.1

Genome annotation databases

EnsemblBacteriaiACA72016; ACA72016; PputW619_1511
KEGGippw:PputW619_1511

Similar proteinsi

Entry informationi

Entry nameiLPXK_PSEPW
AccessioniPrimary (citable) accession number: B1J506
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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