B1J479 (ALGL_PSEPW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alginate lyase
EC=4.2.2.3

Alternative name(s):
Poly(beta-D-mannuronate) lyase
Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PputW619_0882

Organism

Pseudomonas putida (strain W619) [Complete proteome] [HAMAP]

Taxonomic identifier

390235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity. HAMAP MF_00557
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential HAMAP MF_00557.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 367	343	Alginate lyase HAMAP MF_00557		PRO_5000314720

Sequences

	Sequence		Length	Mass (Da)
	B1J479 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 134DA37A99FF6665	FASTA	367	41,551
	10 20 30 40 50 60 MTAFKRIFSP ALLVLALYGG AAHAALVPPQ GYYEGIEKIK TSDGNFRCES APKPFTGALQ 70 80 90 100 110 120 FRSKYEGSDK ARATLNRDSE QAFRDSTKDI TTLERGVAKM VNQYMRDGRP AQLDCTLTWL 130 140 150 160 170 180 GTWARADALM STNYNHTGKS MRKWALGSMS GSWLRLKFSN SQPLAAHQAE AELIEKWFAR 190 200 210 220 230 240 LAEQTVRDWS NLPLEKINNH SYWAAWSVMA TAVATDRRDL FDWAVKEYKV GANQIDDQGF 250 260 270 280 290 300 LPNEIKRKQR ALAYHNYALP PLAMIASFAK ANGVDLRSEN NFALQRLGEG VLYGARDPRH 310 320 330 340 350 360 FAERAGEKQD MKDLKVDGKY AWLEPWCALY QCVGDTLERK HRMQPFDSFR LGGNLTRVYD PSAQSKK

References

[1]

"Complete sequence of Pseudomonas putida W619." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Taghavi S., Vangronsveld D., van der Lelie D., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: W619.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000949 Genomic DNA. Translation: ACA71387.1.
RefSeq	YP_001747756.1. NC_010501.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1J479.
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6109799.
GenomeReviews	Gene locus PputW619_0882 in contig CP000949_GR.
KEGG	ppw:PputW619_0882.
PATRIC	19951495. VBIPsePut93764_0881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG336064.
OMA	AAWSVMA.
ProtClustDB	PRK00325.
Family and domain databases
HAMAP	MF_00557. Alginate_lyase. [Tree]
InterPro	IPR022859. Alginate_lyase. IPR008397. Alginate_lyase_dom. IPR008929. Chondroitin_lyas. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
KO	K01729.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
SUPFAM	SSF48230. Chondroitin_lyas. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEPW

Accession

Primary (citable) accession number: B1J479

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program