Reviewed, UniProtKB/Swiss-Prot B1J390 (HGD_PSEPW)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PputW619_0817

Organism

Pseudomonas putida (strain W619) [Complete proteome] [HAMAP]

Taxonomic identifier

390235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity. HAMAP MF_00334
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_1000119846
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	337	1	Iron By similarity
	Metal binding	367	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1J390-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 577C40745EC024FC	FASTA	433	47,972
	10 20 30 40 50 60 MNRDTSPDLQ YLSGFGNEFA SEALPGALPV GQNSPQKAPY GLYAELLSGT AFTMARSELR 70 80 90 100 110 120 RTWLYRIRPS ALHPRFERLA RQPLNGPLGG VTPNRLRWNP QPLPTEPTDF IEGWLPMVAN 130 140 150 160 170 180 SAADKPAGVS VYIYCANRSM ERVFFNADGE LLIVPEQGRL RIATELGLLE VEPQEIAVVP 190 200 210 220 230 240 RGMKFRVELP DGQARGYIAE NHGAPLRLPD LGPIGSNGLA NPRDFLAPVA HYEEAEGPVQ 250 260 270 280 290 300 LVQKFLGEHW ACELQHSPLD VVAWHGSNVP YKYDLRRFNT LGTVSFDHPD PSIFTVLTSP 310 320 330 340 350 360 TSVHGLANMD FVIFPPRWMV AENTFRPPWF HRNLMNEFMG LISGAYDAKA EGFLPGGASL 370 380 390 400 410 420 HGVMSAHGPD AETCEKAIAA DLAPHKIDNT MAFMFETSQV LRPSLQALEC PQLQADYDSC 430 WATLPSTFNP NRR

References

[1]

"Complete sequence of Pseudomonas putida W619." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Taghavi S., Vangronsveld D., van der Lelie D., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000949 Genomic DNA. Translation: ACA71322.1.
RefSeq	YP_001747691.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6109734.
GenomeReviews	Gene locus PputW619_0817 in contig CP000949_GR.
KEGG	ppw:PputW619_0817.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG293508.
OMA	RNCMSEF.
Family and domain databases
HAMAP	MF_00334. Homogentis_dioxygen. [Tree]
InterPro	IPR011051. Cupin_RmlC_type. IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEPW

Accession

Primary (citable) accession number: B1J390

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)