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B1J2G9

- TRMB_PSEPW

UniProt

B1J2G9 - TRMB_PSEPW

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas putida (strain W619)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701S-adenosyl-L-methionineUniRule annotation
    Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
    Binding sitei122 – 1221S-adenosyl-L-methionineUniRule annotation
    Active sitei145 – 1451By similarity
    Binding sitei145 – 1451S-adenosyl-L-methionineUniRule annotation
    Binding sitei149 – 1491SubstrateUniRule annotation
    Binding sitei181 – 1811SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPPUT390235:GHHJ-363-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:PputW619_0362
    OrganismiPseudomonas putida (strain W619)
    Taxonomic identifieri390235 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000720: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240tRNA (guanine-N(7)-)-methyltransferasePRO_1000136359Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi390235.PputW619_0362.

    Structurei

    3D structure databases

    ProteinModelPortaliB1J2G9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 2214Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiTSATNDY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1J2G9-1 [UniParc]FASTAAdd to Basket

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    MTESQDTPIT TDGEARPHRR IKSFVMRAGR MTEGQQRGLD QGGPLYILPL    50
    AESPVDYDQV FGRSAPRTLE IGFGMGHSLL EMAAAAPEQD FIGVEVHRPG 100
    VGALLNGVLT EGLKNLRVYD CDAIEVLNRC VADNSLDRLM LFFPDPWHKA 150
    RHHKRRIVQL EFAELVRRKL KPGGIFHMAT DWEPYAEYML EVMSAAPGYR 200
    NLAADGAYVP RPEERPITKF ERRGERLGHG VWDLKFEKVG 240
    Length:240
    Mass (Da):27,096
    Last modified:April 29, 2008 - v1
    Checksum:i2177614623DDF4D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA70868.1.
    RefSeqiWP_012312306.1. NC_010501.1.
    YP_001747237.1. NC_010501.1.

    Genome annotation databases

    EnsemblBacteriaiACA70868; ACA70868; PputW619_0362.
    GeneIDi6109142.
    KEGGippw:PputW619_0362.
    PATRICi19950449. VBIPsePut93764_0366.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA70868.1 .
    RefSeqi WP_012312306.1. NC_010501.1.
    YP_001747237.1. NC_010501.1.

    3D structure databases

    ProteinModelPortali B1J2G9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390235.PputW619_0362.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA70868 ; ACA70868 ; PputW619_0362 .
    GeneIDi 6109142.
    KEGGi ppw:PputW619_0362.
    PATRICi 19950449. VBIPsePut93764_0366.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi TSATNDY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci PPUT390235:GHHJ-363-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: W619.

    Entry informationi

    Entry nameiTRMB_PSEPW
    AccessioniPrimary (citable) accession number: B1J2G9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3