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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas putida (strain W619)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701S-adenosyl-L-methionineUniRule annotation
Binding sitei95 – 951S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221S-adenosyl-L-methionineUniRule annotation
Active sitei145 – 1451By similarity
Binding sitei145 – 1451S-adenosyl-L-methionineUniRule annotation
Binding sitei149 – 1491SubstrateUniRule annotation
Binding sitei181 – 1811SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPPUT390235:GHHJ-363-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PputW619_0362
OrganismiPseudomonas putida (strain W619)
Taxonomic identifieri390235 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000720 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240tRNA (guanine-N(7)-)-methyltransferasePRO_1000136359Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi390235.PputW619_0362.

Structurei

3D structure databases

ProteinModelPortaliB1J2G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 2214Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1J2G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESQDTPIT TDGEARPHRR IKSFVMRAGR MTEGQQRGLD QGGPLYILPL
60 70 80 90 100
AESPVDYDQV FGRSAPRTLE IGFGMGHSLL EMAAAAPEQD FIGVEVHRPG
110 120 130 140 150
VGALLNGVLT EGLKNLRVYD CDAIEVLNRC VADNSLDRLM LFFPDPWHKA
160 170 180 190 200
RHHKRRIVQL EFAELVRRKL KPGGIFHMAT DWEPYAEYML EVMSAAPGYR
210 220 230 240
NLAADGAYVP RPEERPITKF ERRGERLGHG VWDLKFEKVG
Length:240
Mass (Da):27,096
Last modified:April 29, 2008 - v1
Checksum:i2177614623DDF4D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA70868.1.
RefSeqiWP_012312306.1. NC_010501.1.
YP_001747237.1. NC_010501.1.

Genome annotation databases

EnsemblBacteriaiACA70868; ACA70868; PputW619_0362.
KEGGippw:PputW619_0362.
PATRICi19950449. VBIPsePut93764_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000949 Genomic DNA. Translation: ACA70868.1.
RefSeqiWP_012312306.1. NC_010501.1.
YP_001747237.1. NC_010501.1.

3D structure databases

ProteinModelPortaliB1J2G9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi390235.PputW619_0362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA70868; ACA70868; PputW619_0362.
KEGGippw:PputW619_0362.
PATRICi19950449. VBIPsePut93764_0366.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiAQHMLEV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciPPUT390235:GHHJ-363-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: W619.

Entry informationi

Entry nameiTRMB_PSEPW
AccessioniPrimary (citable) accession number: B1J2G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: May 27, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.