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B1J1W0

- DAPF_PSEPW

UniProt

B1J1W0 - DAPF_PSEPW

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Pseudomonas putida (strain W619)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei46 – 461SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei159 – 1591SubstrateUniRule annotation
    Sitei161 – 1611Important for catalytic activityUniRule annotation
    Binding sitei192 – 1921SubstrateUniRule annotation
    Sitei210 – 2101Important for catalytic activityUniRule annotation
    Active sitei219 – 2191Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciPPUT390235:GHHJ-244-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:PputW619_0244
    OrganismiPseudomonas putida (strain W619)
    Taxonomic identifieri390235 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000720: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276Diaminopimelate epimerasePRO_1000099257Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 219

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi390235.PputW619_0244.

    Structurei

    3D structure databases

    ProteinModelPortaliB1J1W0.
    SMRiB1J1W0. Positions 3-276.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni210 – 2112Substrate bindingUniRule annotation
    Regioni220 – 2212Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1J1W0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLRFTKMHG LGNDFMVLDL VSQHAHIQPK HAKQWGDRHT GIGFDQLLIV    50
    EAPNNPEVDF RYRIFNADGS EVEQCGNGAR CFARFVLDKR LTAKKRIRVE 100
    TKGGIIELDV QNDGQVCVDM GPPRFIPAEI PFVADEQALN YPLEVDGELH 150
    SIAAVSMGNP HAVLRVDDIR TAPVHGLGPK IENHPRFPQR VNAGFLQVID 200
    RHRANLRVWE RGAGETQACG TGACAAAVAA ISQGWMDSPV SLDLPGGRLN 250
    IEWAGPGTSV LMTGPAVRVY EGQVRL 276
    Length:276
    Mass (Da):30,279
    Last modified:April 29, 2008 - v1
    Checksum:iC33048DE6F81424B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA70750.1.
    RefSeqiWP_012312191.1. NC_010501.1.
    YP_001747119.1. NC_010501.1.

    Genome annotation databases

    EnsemblBacteriaiACA70750; ACA70750; PputW619_0244.
    GeneIDi6109023.
    KEGGippw:PputW619_0244.
    PATRICi19950211. VBIPsePut93764_0248.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA70750.1 .
    RefSeqi WP_012312191.1. NC_010501.1.
    YP_001747119.1. NC_010501.1.

    3D structure databases

    ProteinModelPortali B1J1W0.
    SMRi B1J1W0. Positions 3-276.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390235.PputW619_0244.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA70750 ; ACA70750 ; PputW619_0244 .
    GeneIDi 6109023.
    KEGGi ppw:PputW619_0244.
    PATRICi 19950211. VBIPsePut93764_0248.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci PPUT390235:GHHJ-244-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: W619.

    Entry informationi

    Entry nameiDAPF_PSEPW
    AccessioniPrimary (citable) accession number: B1J1W0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3