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B1J150

- SYL_PSEPW

UniProt

B1J150 - SYL_PSEPW

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Pseudomonas putida (strain W619)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei630 – 6301ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPPUT390235:GHHJ-629-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:PputW619_0627
    OrganismiPseudomonas putida (strain W619)
    Taxonomic identifieri390235 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000720: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 868868Leucine--tRNA ligasePRO_1000091348Add
    BLAST

    Proteomic databases

    PRIDEiB1J150.

    Interactioni

    Protein-protein interaction databases

    STRINGi390235.PputW619_0627.

    Structurei

    3D structure databases

    ProteinModelPortaliB1J150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi627 – 6315"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1J150-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHEQYTPRDI EAAAQHFWDE QQSFAVTEQP GKDTYYCLSM FPYPSGKLHM    50
    GHVRNYTIGD VIARYQRMLG KNVLQPMGWD AFGMPAENAA MKNNVAPAKW 100
    TYENIDYMKT QLKSLGLAID WAREVTTCKP DYYRWEQWLF TRLFEKGIIY 150
    RKNGTVNWDP ADQTVLANEQ VIDGRGWRSG ALIEKREIPM YYFRITDYAD 200
    ELLESLDELP GWPEQVKTMQ RNWIGKSRGM EVQFPYDQAS IGHEGTLKVF 250
    TTRPDTLMGA TYVAVAAEHP LATQAAQGNP ALQAFIDECK SGSVAEADMA 300
    TQEKKGMPTS LLVEHPLTGE KLPVWVANYV LMHYGDGAVM AVPAHDERDF 350
    EFAHKYNLPV KAVVRTSAGD EVGSEWQAAY GEHGQLINSG EFDGLDFAGA 400
    FDAIEAALIR KELGKSRTQF RLRDWGISRQ RYWGCPIPII HCPSCGDVPV 450
    PEDQLPVTLP ENVVPDGAGS PLARMPEFYE CSCPKCGAAA KRETDTMDTF 500
    VESSWYFARY ASPNYDKGLV DPKAANHWLP VDQYIGGIEH AILHLLYARF 550
    FHKLMRDEGL VTSNEPFKNL LTQGMVVAET YYRVASNGGK DWFNPADVEI 600
    ERDAKAKIIG ARLKTDGLPV EIGGTEKMSK SKNNGVDPQS MIEAYGADTC 650
    RLFMMFASPP DMSLEWSDSG VEGASRFLRR VWRLAQGHVN QGLPGKLDLA 700
    ALNDEQKVIR RAIHTAIKQA STDVGQYHKF NTAIAQVMTV MNVLEKAPQS 750
    TEQDRALLQE GLEAVTLLLA PITPHISHEL WKALGHEQAV IDACWPVVDE 800
    SALVQDTVTL VVQVNGKLRG QVEMPAAASR EEIEAAARNN ENVLRFIDGL 850
    TIRKVIVVPG KLVNIVAN 868
    Length:868
    Mass (Da):97,014
    Last modified:April 29, 2008 - v1
    Checksum:i47EC8D7BB190EB97
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA71132.1.
    RefSeqiYP_001747501.1. NC_010501.1.

    Genome annotation databases

    EnsemblBacteriaiACA71132; ACA71132; PputW619_0627.
    GeneIDi6109536.
    KEGGippw:PputW619_0627.
    PATRICi19950985. VBIPsePut93764_0633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000949 Genomic DNA. Translation: ACA71132.1 .
    RefSeqi YP_001747501.1. NC_010501.1.

    3D structure databases

    ProteinModelPortali B1J150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 390235.PputW619_0627.

    Proteomic databases

    PRIDEi B1J150.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA71132 ; ACA71132 ; PputW619_0627 .
    GeneIDi 6109536.
    KEGGi ppw:PputW619_0627.
    PATRICi 19950985. VBIPsePut93764_0633.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci PPUT390235:GHHJ-629-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: W619.

    Entry informationi

    Entry nameiSYL_PSEPW
    AccessioniPrimary (citable) accession number: B1J150
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3