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B1J0J4

- OTSA_ECOLC

UniProt

B1J0J4 - OTSA_ECOLC

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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei77 – 771Glucose-6-phosphateBy similarity
Sitei86 – 861Involved in alpha anomer selectivityBy similarity
Binding sitei131 – 1311Glucose-6-phosphateBy similarity
Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
Binding sitei263 – 2631UDP-glucoseBy similarity
Binding sitei268 – 2681UDP-glucoseBy similarity
Binding sitei301 – 3011Glucose-6-phosphateBy similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciECOL481805:GI3G-1781-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:EcolC_1738
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000317: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348893Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi481805.EcolC_1738.

Structurei

3D structure databases

ProteinModelPortaliB1J0J4.
SMRiB1J0J4. Positions 2-458.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose bindingBy similarity
Regioni366 – 3705UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiWGESFVK.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

B1J0J4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDEHAAS AGGLAVGILG ALKAAGGLWF GWSGETGNED
60 70 80 90 100
QPLKKVKKGN ITWASFNLSE QDLDEYYNQF SNAVLWPAFH YRLDLVQFQR
110 120 130 140 150
PAWDGYLRVN ALLADKLLPL LQDDDIIWIH DYHLLPFAHE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTY DTLLEQLCDY DLLGFQTEND RLAFLDCLSN
210 220 230 240 250
LTRVTTRSAK SHTAWGKAFR TEVYPIGIEP KEIAKQAAGP LPPKLAQLKA
260 270 280 290 300
ELKNVQNIFS VERLDYSKGL PERFLAYEAL LEKYPQHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLENEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKIFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPANP GVLVLSQFAG AANELTSALI
410 420 430 440 450
VNPYDRDEVA AALDRALTMS LAERISRHAE MLDVIVKNDI NHWQECFISD
460 470
LKQIVPRSAE SQQRDKVATF PKLA
Length:474
Mass (Da):53,611
Last modified:April 29, 2008 - v1
Checksum:i63FFB6F938056F95
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000946 Genomic DNA. Translation: ACA77388.1.
RefSeqiYP_001724715.1. NC_010468.1.

Genome annotation databases

EnsemblBacteriaiACA77388; ACA77388; EcolC_1738.
GeneIDi6065352.
KEGGiecl:EcolC_1738.
PATRICi18226286. VBIEscCol82905_1868.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000946 Genomic DNA. Translation: ACA77388.1 .
RefSeqi YP_001724715.1. NC_010468.1.

3D structure databases

ProteinModelPortali B1J0J4.
SMRi B1J0J4. Positions 2-458.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 481805.EcolC_1738.

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA77388 ; ACA77388 ; EcolC_1738 .
GeneIDi 6065352.
KEGGi ecl:EcolC_1738.
PATRICi 18226286. VBIEscCol82905_1868.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191478.
KOi K00697.
OMAi WGESFVK.
OrthoDBi EOG6CCH1M.

Enzyme and pathway databases

UniPathwayi UPA00299 .
BioCyci ECOL481805:GI3G-1781-MONOMER.

Family and domain databases

InterProi IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Escherichia coli C str. ATCC 8739."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Ingram L., Richardson P.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8739 / DSM 1576 / Crooks.

Entry informationi

Entry nameiOTSA_ECOLC
AccessioniPrimary (citable) accession number: B1J0J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: April 29, 2008
Last modified: October 1, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3