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B1IZP1 (GHRB_ECOLC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 31. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyoxylate/hydroxypyruvate reductase B
EC=1.1.1.79
EC=1.1.1.81
Gene names
Name:ghrB
Ordered Locus Names:EcolC_0163
OrganismEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) [Complete proteome] [HAMAP]
Taxonomic identifier481805 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667

Catalytic activity

Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667

D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667

Subunit structure

Homodimer By similarity. HAMAP MF_01667

Subcellular location

Cytoplasm By similarity HAMAP MF_01667.

Sequence similarities

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

glyoxylate reductase (NADP) activity

Inferred from electronic annotation. Source: EC

hydroxypyruvate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667
PRO_0000348383

Sites

Active site2371 By similarity
Active site2661 By similarity
Active site2851Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B1IZP1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: A6F02928A19DD250

FASTA32435,423
        10         20         30         40         50         60 
MKPSVILYKA LPDDLLQRLQ EHFTVHQVAN LSPQTVEQNA AIFAEAEGLL GSNENVDAAL 

        70         80         90        100        110        120 
LEKMPKLRAT STISVGYDNF DVDALTARKI LLMHTPTVLT ETVADTLMAL VLSTARRVVE 

       130        140        150        160        170        180 
VAERVKAGEW TASIGPDWYG TDVHHKTLGI VGMGRIGMAL AQRAHFGFNM PILYNARRHH 

       190        200        210        220        230        240 
KEAEERFNAR YCDLDTLLQE SDFVCLILPL TDETHHLFGA EQFAKMKSSA IFINAGRGPV 

       250        260        270        280        290        300 
VDENALIAAL QKGEIHAAGL DVFEQEPLSV DSPLLSMANV VAVPHIGSAT YETRYGMAAC 

       310        320 
AVDNLIDALQ GKVEKNCVNP HVAD

« Hide

References

[1]"Complete sequence of Escherichia coli C str. ATCC 8739."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Ingram L., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8739 / DSM 1576 / Crooks.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000946 Genomic DNA. Translation: ACA75845.1.
RefSeqYP_001723172.1. NC_010468.1.

3D structure databases

ProteinModelPortalB1IZP1.
SMRB1IZP1. Positions 1-324.
ModBaseSearch...

Protein-protein interaction databases

STRINGB1IZP1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000006146; EBESCP00000005830; EBESCG00000005209.
GeneID6068261.
GenomeReviewsGene locus EcolC_0163 in contig CP000946_GR.
KEGGecl:EcolC_0163.
PATRIC18222808. VBIEscCol82905_0172.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000009079.
HOGENOMHBG731446.
OMAHALTEKK.
ProtClustDBPRK15409.

Enzyme and pathway databases

BioCycECOL481805:ECOLC_0163-MONOMER.

Family and domain databases

HAMAPMF_01667. 2-Hacid_dh_C_GhrB.
[Tree]
InterProIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR006140. D-isomer_2_OHA_DH_NAD-bd.
IPR023756. Glyo/OHPyrv_Rdtase_B.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KOK00090.
PfamPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEPS00065. D_2_HYDROXYACID_DH_1. False negative.
PS00670. D_2_HYDROXYACID_DH_2. 1 hit.
PS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGHRB_ECOLC
AccessionPrimary (citable) accession number: B1IZP1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: April 29, 2008
Last modified: January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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