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B1IZ75

- SYC_ECOLC

UniProt

B1IZ75 - SYC_ECOLC

Protein

Cysteine--tRNA ligase

Gene

cysS

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi28 – 281ZincUniRule annotation
    Metal bindingi209 – 2091ZincUniRule annotation
    Metal bindingi234 – 2341ZincUniRule annotation
    Metal bindingi238 – 2381ZincUniRule annotation
    Binding sitei269 – 2691ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. cysteine-tRNA ligase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteinyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciECOL481805:GI3G-3167-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine--tRNA ligaseUniRule annotation (EC:6.1.1.16UniRule annotation)
    Alternative name(s):
    Cysteinyl-tRNA synthetaseUniRule annotation
    Short name:
    CysRSUniRule annotation
    Gene namesi
    Name:cysSUniRule annotation
    Ordered Locus Names:EcolC_3096
    OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
    Taxonomic identifieri481805 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000317: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461Cysteine--tRNA ligasePRO_1000074612Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi481805.EcolC_3096.

    Structurei

    3D structure databases

    ProteinModelPortaliB1IZ75.
    SMRiB1IZ75. Positions 1-461.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi30 – 4011"HIGH" regionAdd
    BLAST
    Motifi266 – 2705"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0215.
    HOGENOMiHOG000245250.
    KOiK01883.
    OMAiMHADFDA.
    OrthoDBiEOG6RVFXC.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00041. Cys_tRNA_synth.
    InterProiIPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR10890. PTHR10890. 1 hit.
    PfamiPF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view]
    PRINTSiPR00983. TRNASYNTHCYS.
    SMARTiSM00840. DALR_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    TIGRFAMsiTIGR00435. cysS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1IZ75-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV    50
    ARYLRFLGYK LKYVRNITDI DDKIIKRANE NGESFVALVD RMIAEMHKDF 100
    DALNILRPDM EPRATHHIAE IIELTEQLIA KGHAYVADNG DVMFDVPTDP 150
    TYGVLSRQDL DQLQAGARVD VVDDKRNPMD FVLWKMSKEG EPSWPSPWGA 200
    GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA QSTCAHDGQY 250
    VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS 300
    QLNYSEENLK QARAALERLY TALRGTDKTV APAGGEAFEA RFIEAMDDDF 350
    NTPEAYSVLF DMAREVNRLK AEDMAAANAM ASHLRKLSAV LGLLEQEPEA 400
    FLQSGAQADD SEVAEIEALI QQRLDARKAK DWAAADAARD RLNEMGIVLE 450
    DGPQGTTWRR K 461
    Length:461
    Mass (Da):52,184
    Last modified:April 29, 2008 - v1
    Checksum:iDFA73ADB42C58885
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000946 Genomic DNA. Translation: ACA78720.1.
    RefSeqiYP_001726047.1. NC_010468.1.

    Genome annotation databases

    EnsemblBacteriaiACA78720; ACA78720; EcolC_3096.
    GeneIDi6066247.
    KEGGiecl:EcolC_3096.
    PATRICi18229202. VBIEscCol82905_3304.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000946 Genomic DNA. Translation: ACA78720.1 .
    RefSeqi YP_001726047.1. NC_010468.1.

    3D structure databases

    ProteinModelPortali B1IZ75.
    SMRi B1IZ75. Positions 1-461.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 481805.EcolC_3096.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA78720 ; ACA78720 ; EcolC_3096 .
    GeneIDi 6066247.
    KEGGi ecl:EcolC_3096.
    PATRICi 18229202. VBIEscCol82905_3304.

    Phylogenomic databases

    eggNOGi COG0215.
    HOGENOMi HOG000245250.
    KOi K01883.
    OMAi MHADFDA.
    OrthoDBi EOG6RVFXC.

    Enzyme and pathway databases

    BioCyci ECOL481805:GI3G-3167-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00041. Cys_tRNA_synth.
    InterProi IPR015803. Cys-tRNA-ligase.
    IPR015273. Cys-tRNA-synt_Ia_DALR.
    IPR024909. Cys-tRNA/MSH_ligase.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR10890. PTHR10890. 1 hit.
    Pfami PF09190. DALR_2. 1 hit.
    PF01406. tRNA-synt_1e. 1 hit.
    [Graphical view ]
    PRINTSi PR00983. TRNASYNTHCYS.
    SMARTi SM00840. DALR_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    TIGRFAMsi TIGR00435. cysS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Escherichia coli C str. ATCC 8739."
      Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
      , Kyrpides N., Mikhailova N., Ingram L., Richardson P.
      Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8739 / DSM 1576 / Crooks.

    Entry informationi

    Entry nameiSYC_ECOLC
    AccessioniPrimary (citable) accession number: B1IZ75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3