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B1IXX9 (GAL1_ECOLC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactokinase

EC=2.7.1.6
Alternative name(s):
Galactose kinase
Gene names
Name:galK
Ordered Locus Names:EcolC_2905
OrganismEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) [Complete proteome] [HAMAP]
Taxonomic identifier481805 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) By similarity. HAMAP-Rule MF_00246

Catalytic activity

ATP + alpha-D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP-Rule MF_00246

Pathway

Carbohydrate metabolism; galactose metabolism. HAMAP-Rule MF_00246

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00246.

Sequence similarities

Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Galactose metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

galactokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Galactokinase HAMAP-Rule MF_00246
PRO_1000078407

Regions

Nucleotide binding124 – 1307ATP By similarity
Region34 – 374Substrate binding By similarity

Sites

Active site1741Proton acceptor By similarity
Metal binding1301Magnesium By similarity
Metal binding1621Magnesium By similarity
Binding site2231Substrate By similarity
Site281Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
B1IXX9 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 7D886CE6FC48F0A1

FASTA38241,442
        10         20         30         40         50         60 
MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD 

        70         80         90        100        110        120 
DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN 

       130        140        150        160        170        180 
VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL 

       190        200        210        220        230        240 
GKKDHALLID CRSLGTKAVS MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP 

       250        260        270        280        290        300 
ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS 

       310        320        330        340        350        360 
MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP AVQQAVAEQY 

       370        380 
EAKTGIKETF YVCKPSQGAG QC 

« Hide

References

[1]"Complete sequence of Escherichia coli C str. ATCC 8739."
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Ingram L., Richardson P.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 8739 / DSM 1576 / Crooks.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000946 Genomic DNA. Translation: ACA78532.1.
RefSeqYP_001725859.1. NC_010468.1.

3D structure databases

ProteinModelPortalB1IXX9.
SMRB1IXX9. Positions 11-379.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING481805.EcolC_2905.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA78532; ACA78532; EcolC_2905.
GeneID6065384.
KEGGecl:EcolC_2905.
PATRIC18228764. VBIEscCol82905_3100.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0153.
HOGENOMHOG000241100.
KOK00849.
OMAYNDGFVM.
OrthoDBEOG6DG2SH.
ProtClustDBPRK05101.

Enzyme and pathway databases

BioCycECOL481805:GI3G-2961-MONOMER.
UniPathwayUPA00214.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00246. Galactokinase.
InterProIPR000705. Galactokinase.
IPR022963. Galactokinase_bac.
IPR019741. Galactokinase_CS.
IPR019539. GalKase_gal-bd.
IPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR006203. GHMP_knse_ATP-bd_CS.
IPR006206. Mevalonate/galactokinase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamPF10509. GalKase_gal_bdg. 1 hit.
PF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF000530. Galactokinase. 1 hit.
PRINTSPR00473. GALCTOKINASE.
PR00959. MEVGALKINASE.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00131. gal_kin. 1 hit.
PROSITEPS00106. GALACTOKINASE. 1 hit.
PS00627. GHMP_KINASES_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAL1_ECOLC
AccessionPrimary (citable) accession number: B1IXX9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways