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Protein

2-keto-3-deoxy-L-rhamnonate aldolase

Gene

rhmA

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible retro-aldol cleavage of 2-keto-3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde.UniRule annotation

Catalytic activityi

2-dehydro-3-deoxy-L-rhamnonate = pyruvate + (S)-lactaldehyde.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei49 – 491Proton acceptorUniRule annotation
Sitei74 – 741Transition state stabilizerUniRule annotation
Sitei88 – 881Increases basicity of active site HisUniRule annotation
Binding sitei151 – 1511SubstrateUniRule annotation
Metal bindingi153 – 1531MagnesiumUniRule annotation
Binding sitei178 – 1781Substrate; via amide nitrogenUniRule annotation
Metal bindingi179 – 1791MagnesiumUniRule annotation
Binding sitei179 – 1791SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL481805:GI3G-1442-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2-keto-3-deoxy-L-rhamnonate aldolaseUniRule annotation (EC:4.1.2.53UniRule annotation)
Short name:
KDR aldolaseUniRule annotation
Alternative name(s):
2-dehydro-3-deoxyrhamnonate aldolaseUniRule annotation
Gene namesi
Name:rhmAUniRule annotation
Ordered Locus Names:EcolC_1404
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2672672-keto-3-deoxy-L-rhamnonate aldolasePRO_0000353169Add
BLAST

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Protein-protein interaction databases

STRINGi481805.EcolC_1404.

Structurei

3D structure databases

ProteinModelPortaliB1IXU2.
SMRiB1IXU2. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNV. Bacteria.
COG3836. LUCA.
HOGENOMiHOG000179750.
KOiK12660.
OMAiIARASQW.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_01290. KDR_aldolase. 1 hit.
InterProiIPR005000. Aldolase/citrate-lyase_domain.
IPR023593. KDR_aldolase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF03328. HpcH_HpaI. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.

Sequencei

Sequence statusi: Complete.

B1IXU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNALLSNPFK ERLRKGEVQI GLWLSSTTAY MAEIAATSGY DWLLIDGEHA
60 70 80 90 100
PNTIQDLYHQ LQAVAPYASQ PVIRPVEGSK PLIKQVLDIG AQTLLIPMVD
110 120 130 140 150
TAEQARQVVS ATRYPPYGER GVGASVARAA RWGRIENYMA QVNDSLCLLV
160 170 180 190 200
QVESKTALDN LDEILDVEGI DGVFIGPADL SASLGYPDNA GHPEVQRIIE
210 220 230 240 250
TSIRRIRAAG KAAGFLAVAP DMAQQCLAWG ANFVAVGVDT MLYSDALDQR
260
LAMFKSGKNG PRIKGSY
Length:267
Mass (Da):28,916
Last modified:April 29, 2008 - v1
Checksum:iF68506D8A11D23FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA77068.1.
RefSeqiWP_000992954.1. NC_010468.1.

Genome annotation databases

EnsemblBacteriaiACA77068; ACA77068; EcolC_1404.
KEGGiecl:EcolC_1404.
PATRICi18225529. VBIEscCol82905_1499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA77068.1.
RefSeqiWP_000992954.1. NC_010468.1.

3D structure databases

ProteinModelPortaliB1IXU2.
SMRiB1IXU2. Positions 1-256.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi481805.EcolC_1404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA77068; ACA77068; EcolC_1404.
KEGGiecl:EcolC_1404.
PATRICi18225529. VBIEscCol82905_1499.

Phylogenomic databases

eggNOGiENOG4105CNV. Bacteria.
COG3836. LUCA.
HOGENOMiHOG000179750.
KOiK12660.
OMAiIARASQW.

Enzyme and pathway databases

BioCyciECOL481805:GI3G-1442-MONOMER.

Family and domain databases

Gene3Di3.20.20.60. 1 hit.
HAMAPiMF_01290. KDR_aldolase. 1 hit.
InterProiIPR005000. Aldolase/citrate-lyase_domain.
IPR023593. KDR_aldolase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF03328. HpcH_HpaI. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRHMA_ECOLC
AccessioniPrimary (citable) accession number: B1IXU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: April 29, 2008
Last modified: September 7, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.