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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi53Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi82Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi150Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi154Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi157Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotation (EC:2.8.4.4UniRule annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotation
Ordered Locus Names:EcolC_2807
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003748171 – 441Ribosomal protein S12 methylthiotransferase RimOAdd BLAST441

Structurei

3D structure databases

ProteinModelPortaliB1IXD2
SMRiB1IXD2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 118MTTase N-terminalUniRule annotationAdd BLAST111
Domaini376 – 441TRAMUniRule annotationAdd BLAST66

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CBM Bacteria
COG0621 LUCA
HOGENOMiHOG000224766
KOiK14441
OMAiCLSERYK

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

B1IXD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVTPQPKI GFVSLGCPKN LVDSERILTE LRTEGYDVVP SYDDADMVIV
60 70 80 90 100
NTCGFIDSAV QESLEAIGEA LNENGKVIVT GCLGAKEDQI REVHPKVLEI
110 120 130 140 150
TGPHSYEQVL EHVHHYVPKP KHNPFLSLVP EQGVKLTPRH YAYLKISEGC
160 170 180 190 200
NHRCTFCIIP SMRGDLVSRP IGEVLSEAKR LVDAGVKEIL VISQDTSAYG
210 220 230 240 250
VDVKHRTGFH NGEPVKTSMV SLCEQLSKLG IWTRLHYVYP YPHVDDVIPL
260 270 280 290 300
MAEGKILPYL DIPLQHASPR ILKLMKRPGS VDRQLARIKQ WREICPELTL
310 320 330 340 350
RSTFIVGFPG ETEEDFQMLL DFLKEARLDR VGCFKYSPVE GADANALPDQ
360 370 380 390 400
VPEEVKEERW NRFMQLQQQI SAERLQEKVG REILVIIDEV DEEGAIGRSM
410 420 430 440
ADAPEIDGAV YLNGETNVKP GDILRVKVEH ADEYDLWGSR V
Length:441
Mass (Da):49,582
Last modified:April 29, 2008 - v1
Checksum:iE1D4605388AFB889
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA Translation: ACA78435.1
RefSeqiWP_000049367.1, NC_010468.1

Genome annotation databases

EnsemblBacteriaiACA78435; ACA78435; EcolC_2807
KEGGiecl:EcolC_2807

Similar proteinsi

Entry informationi

Entry nameiRIMO_ECOLC
AccessioniPrimary (citable) accession number: B1IXD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: April 29, 2008
Last modified: March 28, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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