B1IXD2 (RIMO_ECOLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal protein S12 methylthiotransferase RimO
Short name=S12 MTTase
Short name=S12 methylthiotransferase
EC=2.-.-.-

Alternative name(s):
Ribosome maturation factor RimO

Gene names

Name:	rimO
Ordered Locus Names:	EcolC_2807

Organism

Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) [Complete proteome] [HAMAP]

Taxonomic identifier

481805 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli C

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 By similarity. HAMAP-Rule MF_01865
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01865.
Sequence similarities	Belongs to the methylthiotransferase family. RimO subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA modification Inferred from electronic annotation. Source: InterPro peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 441	441	Ribosomal protein S12 methylthiotransferase RimO HAMAP-Rule MF_01865		PRO_0000374817
Regions
	Domain	8 – 118	111	MTTase N-terminal
	Domain	376 – 441	66	TRAM
Sites
	Metal binding	17	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	82	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	150	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	154	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	157	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1IXD2 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: E1D4605388AFB889	FASTA	441	49,582
	10 20 30 40 50 60 MSKVTPQPKI GFVSLGCPKN LVDSERILTE LRTEGYDVVP SYDDADMVIV NTCGFIDSAV 70 80 90 100 110 120 QESLEAIGEA LNENGKVIVT GCLGAKEDQI REVHPKVLEI TGPHSYEQVL EHVHHYVPKP 130 140 150 160 170 180 KHNPFLSLVP EQGVKLTPRH YAYLKISEGC NHRCTFCIIP SMRGDLVSRP IGEVLSEAKR 190 200 210 220 230 240 LVDAGVKEIL VISQDTSAYG VDVKHRTGFH NGEPVKTSMV SLCEQLSKLG IWTRLHYVYP 250 260 270 280 290 300 YPHVDDVIPL MAEGKILPYL DIPLQHASPR ILKLMKRPGS VDRQLARIKQ WREICPELTL 310 320 330 340 350 360 RSTFIVGFPG ETEEDFQMLL DFLKEARLDR VGCFKYSPVE GADANALPDQ VPEEVKEERW 370 380 390 400 410 420 NRFMQLQQQI SAERLQEKVG REILVIIDEV DEEGAIGRSM ADAPEIDGAV YLNGETNVKP 430 440 GDILRVKVEH ADEYDLWGSR V

References

[1]

"Complete sequence of Escherichia coli C str. ATCC 8739." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Ingram L., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8739 / DSM 1576 / Crooks.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000946 Genomic DNA. Translation: ACA78435.1.
RefSeq	YP_001725762.1. NC_010468.1.
3D structure databases
ProteinModelPortal	B1IXD2.
SMR	B1IXD2. Positions 139-441.
ModBase	Search...
Protein-protein interaction databases
STRING	481805.EcolC_2807.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA78435; ACA78435; EcolC_2807.
GeneID	6065001.
KEGG	ecl:EcolC_2807.
PATRIC	18228570. VBIEscCol82905_3003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224766.
KO	K14441.
OMA	CPDITLR.
ProtClustDB	PRK14862.
Enzyme and pathway databases
BioCyc	ECOL481805:GI3G-2863-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.80.30.20. 1 hit.
HAMAP	MF_01865. MTTase_RimO.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR012340. NA-bd_OB-fold. IPR005840. Ribosomal_S12_MeSTrfase_RimO. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00089. TIGR00089. 1 hit. TIGR01125. TIGR01125. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMO_ECOLC

Accession

Primary (citable) accession number: B1IXD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	April 29, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program