B1IW72 (UBIE_ECOLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinone/menaquinone biosynthesis methyltransferase ubiE
EC=2.1.1.163
EC=2.1.1.201

Alternative name(s):
2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
Demethylmenaquinone methyltransferase

Gene names

Name:	ubiE
Ordered Locus Names:	EcolC_4175

Organism

Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) [Complete proteome] [HAMAP]

Taxonomic identifier

481805 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli C

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) By similarity. HAMAP-Rule MF_01813
Catalytic activity	A demethylmenaquinone + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine. HAMAP-Rule MF_01813 S-adenosyl-L-methionine + 2-methoxy-6-all-trans-polyprenyl-1,4-benzoquinol = S-adenosyl-L-homocysteine + 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol. HAMAP-Rule MF_01813
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 8/8. HAMAP-Rule MF_01813 Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP-Rule MF_01813
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis Ubiquinone biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway ubiquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Ubiquinone/menaquinone biosynthesis methyltransferase ubiE HAMAP-Rule MF_01813		PRO_1000088282

Sequences

	Sequence		Length	Mass (Da)
	B1IW72 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: B1EEE209BDC68F5E	FASTA	251	28,073
	10 20 30 40 50 60 MVDKSQETTH FGFQTVAKEQ KADMVAHVFH SVASKYDVMN DLMSFGIHRL WKRFTIDCSG 70 80 90 100 110 120 VRRGQTVLDL AGGTGDLTAK FSRLVGETGK VVLADINESM LKMGREKLRN IGVIGNVEYV 130 140 150 160 170 180 QANAEALPFP DNTFDCITIS FGLRNVTDKD KALRSMYRVL KPGGRLLVLE FSKPIIEPLS 190 200 210 220 230 240 KAYDAYSFHV LPRIGSLVAN DADSYRYLAE SIRMHPDQDT LKAMMQDAGF ESVDYYNLTA 250 GVVALHRGYK F

References

[1]

"Complete sequence of Escherichia coli C str. ATCC 8739." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Ingram L., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8739 / DSM 1576 / Crooks.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000946 Genomic DNA. Translation: ACA79772.1.
RefSeq	YP_001727099.1. NC_010468.1.
3D structure databases
ProteinModelPortal	B1IW72.
SMR	B1IW72. Positions 29-231.
ModBase	Search...
Protein-protein interaction databases
STRING	481805.EcolC_4175.
Proteomic databases
PRIDE	B1IW72.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA79772; ACA79772; EcolC_4175.
GeneID	6067259.
KEGG	ecl:EcolC_4175.
PATRIC	18231615. VBIEscCol82905_4476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2226.
HOGENOM	HOG000249463.
KO	K03183.
OMA	PYQYLVE.
ProtClustDB	PRK00216.
Enzyme and pathway databases
BioCyc	ECOL481805:GI3G-4282-MONOMER.
UniPathway	UPA00079; UER00169. UPA00232.
Family and domain databases
HAMAP	MF_01813. UbiE_methyltran.
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. IPR025765. UbiE/COQ5_MeTrFase_dom. [Graphical view]
PANTHER	PTHR10108:SF24. PTHR10108:SF24. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIE_ECOLC

Accession

Primary (citable) accession number: B1IW72

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 29, 2008
Last modified:	May 29, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program