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Protein

ATP-dependent 6-phosphofructokinase isozyme 1

Gene

pfkA

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase isozyme 1 (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12ATP; via amide nitrogenUniRule annotation1
Metal bindingi104Magnesium; catalyticUniRule annotation1
Active sitei128Proton acceptorUniRule annotation1
Binding sitei155Allosteric activator ADPUniRule annotation1
Binding sitei163Substrate; shared with dimeric partnerUniRule annotation1
Binding sitei212Allosteric activator ADPUniRule annotation1
Binding sitei223SubstrateUniRule annotation1
Binding sitei244Substrate; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 74ATPUniRule annotation2
Nucleotide bindingi103 – 106ATPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase isozyme 1UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 1UniRule annotation
Short name:
Phosphofructokinase 1UniRule annotation
Alternative name(s):
6-phosphofructokinase isozyme I
Phosphohexokinase 1UniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:EcolC_4102
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000793091 – 320ATP-dependent 6-phosphofructokinase isozyme 1Add BLAST320

Proteomic databases

PRIDEiB1IVG3.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi481805.EcolC_4102.

Structurei

3D structure databases

ProteinModelPortaliB1IVG3.
SMRiB1IVG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 26Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation5
Regioni55 – 60Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation6
Regioni126 – 128Substrate bindingUniRule annotation3
Regioni170 – 172Substrate bindingUniRule annotation3
Regioni186 – 188Allosteric activator ADP bindingUniRule annotation3
Regioni214 – 216Allosteric activator ADP bindingUniRule annotation3
Regioni250 – 253Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiAIITICE.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1IVG3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKIGVLTS GGDAPGMNAA IRGVVRSALT EGLEVMGIYD GYLGLYEDRM
60 70 80 90 100
VQLDRYSVSD MINRGGTFLG SARFPEFRDE NIRAVAIENL KKRGIDALVV
110 120 130 140 150
IGGDGSYMGA MRLTEMGFPC IGLPGTIDND IKGTDYTIGF FTALSTVVEA
160 170 180 190 200
IDRLRDTSSS HQRISVVEVM GRYCGDLTLA AAIAGGCEFV VVPEVEFSRE
210 220 230 240 250
DLVNEIKAGI AKGKKHAIVA ITEHMCDVDE LAHFIEKETG RETRATVLGH
260 270 280 290 300
IQRGGSPVPY DRILASRMGA YAIDLLLAGY GGRCVGIQNE QLVHHDIIDA
310 320
IENMKRPFKG DWLDCAKKLY
Length:320
Mass (Da):34,842
Last modified:April 29, 2008 - v1
Checksum:iD03D79F6A5536A41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA79700.1.
RefSeqiWP_000591795.1. NC_010468.1.

Genome annotation databases

EnsemblBacteriaiACA79700; ACA79700; EcolC_4102.
KEGGiecl:EcolC_4102.
PATRICi18231443. VBIEscCol82905_4395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA79700.1.
RefSeqiWP_000591795.1. NC_010468.1.

3D structure databases

ProteinModelPortaliB1IVG3.
SMRiB1IVG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi481805.EcolC_4102.

Proteomic databases

PRIDEiB1IVG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA79700; ACA79700; EcolC_4102.
KEGGiecl:EcolC_4102.
PATRICi18231443. VBIEscCol82905_4395.

Phylogenomic databases

eggNOGiENOG4105CTQ. Bacteria.
COG0205. LUCA.
HOGENOMiHOG000248870.
KOiK00850.
OMAiAIITICE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

CDDicd00763. Bacterial_PFK. 1 hit.
HAMAPiMF_00339. Phosphofructokinase_I_B1. 1 hit.
InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP_prok.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_ECOLC
AccessioniPrimary (citable) accession number: B1IVG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 29, 2008
Last modified: November 30, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.