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Protein

Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB

Gene

rutB

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby releasing one of the pyrimidine rings nitrogen atoms as ammonia and one of its carbons as CO2.UniRule annotation

Catalytic activityi

(Z)-3-ureidoacrylate peracid + H2O = (Z)-3-peroxyaminoacrylate + CO2 + NH3.UniRule annotation
(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + CO2 + NH3.UniRule annotation
(Z)-2-methyl-ureidoacrylate peracid + H2O = (Z)-2-methyl-peroxyaminoacrylate + CO2 + NH3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBUniRule annotation (EC:3.5.1.110UniRule annotation)
Alternative name(s):
Ureidoacrylate amidohydrolaseUniRule annotation
Gene namesi
Name:rutBUniRule annotation
Ordered Locus Names:EcolC_2584
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004026581 – 231Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutBAdd BLAST231

Expressioni

Inductioni

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.UniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi481805.EcolC_2584.

Structurei

3D structure databases

ProteinModelPortaliB1IV86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the isochorismatase family. RutB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1IV86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTTLTARPE AITFDPQQSA LIVVDMQNAY ATPGGYLDLA GFDVSTTRPV
60 70 80 90 100
IANIQTAVTA ARAAGMLIIW FQNGWDEQYV EAGGPGSPNF HKSNALKTMR
110 120 130 140 150
KQPQLQGKLL AKGSWDYQLV DELVPQPGDI VLPKPRYSGF FNTPLDSILR
160 170 180 190 200
SRGIRHLVFT GIATNVCVES TLRDGFFLEY FGVVLEDATH QAGPEFAQKA
210 220 230
ALFNIETFFG WVSDVETFCD ALSPTSFARI A
Length:231
Mass (Da):25,360
Last modified:April 29, 2008 - v1
Checksum:i4ACA2AB70CD588AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA78215.1.

Genome annotation databases

EnsemblBacteriaiACA78215; ACA78215; EcolC_2584.
KEGGiecl:EcolC_2584.
PATRICi18228096. VBIEscCol82905_2768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA78215.1.

3D structure databases

ProteinModelPortaliB1IV86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi481805.EcolC_2584.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA78215; ACA78215; EcolC_2584.
KEGGiecl:EcolC_2584.
PATRICi18228096. VBIEscCol82905_2768.

Phylogenomic databases

eggNOGiENOG4106XMA. Bacteria.
COG1335. LUCA.
HOGENOMiHOG000078669.
KOiK09020.
OMAiLAKGGWD.

Family and domain databases

Gene3Di3.40.50.850. 1 hit.
HAMAPiMF_00830. RutB. 1 hit.
InterProiIPR019916. IsoChorase_RutB.
IPR000868. Isochorismatase-like.
[Graphical view]
PfamiPF00857. Isochorismatase. 1 hit.
[Graphical view]
SUPFAMiSSF52499. SSF52499. 1 hit.
TIGRFAMsiTIGR03614. RutB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUTB_ECOLC
AccessioniPrimary (citable) accession number: B1IV86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.