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Protein

Thiamine kinase

Gene

thiK

Organism
Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of thiamine to thiamine phosphate.UniRule annotation

Catalytic activityi

ATP + thiamine = ADP + thiamine phosphate.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from thiamine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine kinase (thiK)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL481805:GI3G-2548-MONOMER.
UniPathwayiUPA00060; UER00596.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine kinaseUniRule annotation (EC:2.7.1.89UniRule annotation)
Gene namesi
Name:thiKUniRule annotation
Ordered Locus Names:EcolC_2495
OrganismiEscherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)
Taxonomic identifieri481805 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Thiamine kinasePRO_1000088027Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi481805.EcolC_2495.

Structurei

3D structure databases

ProteinModelPortaliB1IUF9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RUM. Bacteria.
COG0510. LUCA.
HOGENOMiHOG000219316.
KOiK07251.
OMAiDYLVLMW.
OrthoDBiEOG63NMG9.

Family and domain databases

HAMAPiMF_01604. Thiamine_kinase.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR014093. Thiamine_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR02721. ycfN_thiK. 1 hit.

Sequencei

Sequence statusi: Complete.

B1IUF9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFRSNNPIT RDELLSRFFP QFHPVTTFNS GLSGGSFLIE HQGQRFVVRQ
60 70 80 90 100
PHDPDAPQSA FLRQYRALSQ LPACIAPKPH LYLRDWMVVD YLPGAVKTYL
110 120 130 140 150
PDTNELAGLL YYLHQQPRFG WRITLLPLLE LYWQQSDPAR RTVGWLRMLK
160 170 180 190 200
RLRKAREPRP LRLSPLHMDV HAGNLVHSAS GLKLIDWEYA GDGDIALELA
210 220 230 240 250
AVWVENTEQH LQLVNDYATR AKIYPAQLWR QVRRWFPWLL MLKAGWFEYR
260 270
WRQTGDQQFI RLADDTWRQL LIKQ
Length:274
Mass (Da):32,338
Last modified:April 29, 2008 - v1
Checksum:i165EAF75D39A44D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA78126.1.
RefSeqiWP_001116565.1. NC_010468.1.

Genome annotation databases

EnsemblBacteriaiACA78126; ACA78126; EcolC_2495.
KEGGiecl:EcolC_2495.
PATRICi18227892. VBIEscCol82905_2667.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000946 Genomic DNA. Translation: ACA78126.1.
RefSeqiWP_001116565.1. NC_010468.1.

3D structure databases

ProteinModelPortaliB1IUF9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi481805.EcolC_2495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA78126; ACA78126; EcolC_2495.
KEGGiecl:EcolC_2495.
PATRICi18227892. VBIEscCol82905_2667.

Phylogenomic databases

eggNOGiENOG4108RUM. Bacteria.
COG0510. LUCA.
HOGENOMiHOG000219316.
KOiK07251.
OMAiDYLVLMW.
OrthoDBiEOG63NMG9.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00596.
BioCyciECOL481805:GI3G-2548-MONOMER.

Family and domain databases

HAMAPiMF_01604. Thiamine_kinase.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR014093. Thiamine_kinase.
[Graphical view]
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR02721. ycfN_thiK. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Escherichia coli C str. ATCC 8739."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.
    , Kyrpides N., Mikhailova N., Ingram L., Richardson P.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8739 / DSM 1576 / Crooks.

Entry informationi

Entry nameiTHIK_ECOLC
AccessioniPrimary (citable) accession number: B1IUF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: April 29, 2008
Last modified: December 9, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.