Reviewed, UniProtKB/Swiss-Prot B1IUE6 (NAGK_ECOLC)

Names and origin

Protein names

Recommended name:
    N-acetyl-D-glucosamine kinase
    EC=2.7.1.59
Alternative name(s):
    GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	EcolC_2482

Organism

Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) [Complete proteome] [HAMAP]

Taxonomic identifier

481805 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity.
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_01271
Sequence similarities	Belongs to the ROK (nagC/xylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	N-acetyl-D-glucosamine kinase HAMAP MF_01271		PRO_1000085841
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential
Sites
	Metal binding	157	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	179	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1IUE6-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: B957E62825894BBD	FASTA	303	33,013
	10 20 30 40 50 60 MYYGFDIGGT KIALGVFDSG RQLQWEKRVP TPRDSYDAFL DAVCELVAEA DQRFGCKGSV 70 80 90 100 110 120 GIGIPGMPET EDGTLYAANV PAASGKPLRA DLSARLDRDV RLDNDANCFA LSEAWDDEFT 130 140 150 160 170 180 QYPLVMGLIL GTGVGGGLIF NGKPITGKSY ITGEFGHMRL PVDALTMMGL DFPLRRCGCG 190 200 210 220 230 240 QHGCIENYLS GRGFAWLYQH YYHQPLQAPE IIALYDQGDE QARAHVERYL DLLAVCLGNI 250 260 270 280 290 300 LTIVDPDLVV IGGGLSNFPA ITAQLADRLP RHLLPVARVP RIERARHGDA GGMRGAAFLH LTD

References

[1]

"Complete sequence of Escherichia coli C str. ATCC 8739." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Ingram L., Richardson P. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000946 Genomic DNA. Translation: ACA78113.1.
RefSeq	YP_001725440.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6065563.
GenomeReviews	Gene locus EcolC_2482 in contig CP000946_GR.
KEGG	ecl:EcolC_2482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B1IUE6. HVERFME.
Family and domain databases
HAMAP	MF_01271. [Tree]
InterPro	IPR000600. ROK. [Graphical view]
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGK_ECOLC

Accession

Primary (citable) accession number: B1IUE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 29, 2008
Last modified:	June 16, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)