B1ILA9 (HIS8_CLOBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidinol-phosphate aminotransferase
EC=2.6.1.9

Alternative name(s):
Imidazole acetol-phosphate transaminase

Gene names

Name:	hisC
Ordered Locus Names:	CLD_2981

Organism

Clostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]

Taxonomic identifier

498213 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate. HAMAP MF_01023
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01023
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9. HAMAP MF_01023
Subunit structure	Homodimer By similarity. HAMAP MF_01023
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-phenylalanine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC histidinol-phosphate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Histidinol-phosphate aminotransferase HAMAP MF_01023		PRO_1000135389
Amino acid modifications
	Modified residue	210	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1ILA9 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 6998A636A2DFEE86	FASTA	354	40,585
	10 20 30 40 50 60 MSKYWSNITK NIEPYVCGEQ PKNKKIIKLN TNENPYPPSP KVLQAIKNAA RDDLRLYPDP 70 80 90 100 110 120 NCDALRKTIA NYYNLSKEEV FIGNGSDEVL ALSFLTFFNP EETVVFSDIS YSFYPVYANL 130 140 150 160 170 180 YKLDYKLAKI REDFSIDIND FKNARGGAVI TNPNAPTGVY LSLDSIKQIL EDNVNKVVIV 190 200 210 220 230 240 DEAYIDFGGE SSVNLIKDYP NVLVIQTLSK SRSLAGMRIG FALGQKELIK GLNRIKNSFN 250 260 270 280 290 300 SYTIDRISSL AAIEAIKDEE YFKKCTSKVI KTRNWTINEL RKIGFKIIPS KANFIFITHD 310 320 330 340 350 TYQAEDIFIK LKDENVLVRY FNKDRISNYL RVSIGSKEEM EIFMDKIKKI INKL

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Okra / Type B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000939 Genomic DNA. Translation: ACA45479.1.
RefSeq	YP_001781207.1. NC_010516.1.
3D structure databases
ProteinModelPortal	B1ILA9.
ModBase	Search...
Protein-protein interaction databases
STRING	B1ILA9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6149250.
GenomeReviews	Gene locus CLD_2981 in contig CP000939_GR.
KEGG	cbb:CLD_2981.
PATRIC	19403632. VBICloBot127283_1720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646350.
OMA	MRGELND.
ProtClustDB	PRK05387.
Enzyme and pathway databases
BioCyc	CBOT498213:CLD_2981-MONOMER.
Family and domain databases
HAMAP	MF_01023. HisC_aminotrans_2. [Tree]
InterPro	IPR001917. Aminotrans_II_pyridoxalP_BS. IPR004839. Aminotransferase_I/II. IPR005861. HisP_aminotrans. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00817.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01141. HisC. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS8_CLOBK

Accession

Primary (citable) accession number: B1ILA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program