Reviewed, UniProtKB/Swiss-Prot B1IL94 (GCH1_CLOBK)

Names and origin

Protein names

Recommended name:
    GTP cyclohydrolase 1
    EC=3.5.4.16
Alternative name(s):
    GTP cyclohydrolase I
      Short name=GTP-CH-I

Gene names

Name:	folE
Ordered Locus Names:	CLD_2996

Organism

Clostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]

Taxonomic identifier

498213 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP MF_00223
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP MF_00223
Subunit structure	Homopolymer By similarity. HAMAP MF_00223
Sequence similarities	Belongs to the GTP cyclohydrolase I family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	GTP cyclohydrolase 1 HAMAP MF_00223		PRO_1000100167
Sites
	Metal binding	86	1	Zinc By similarity
	Metal binding	89	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1IL94-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 1FC1FF1818071589	FASTA	196	22,270
	10 20 30 40 50 60 MAIDVKAIEE HIRGILIALG DNPEREGLKN TPKRVAKMYE EVFKGMCYSN DEIAEMFNVT 70 80 90 100 110 120 FEDDLCINDN ENDMVFMKEI EIFSHCEHHL ALMYNMKVAI AYIPKKKIIG LSKIARIADM 130 140 150 160 170 180 VGRRLQLQER IGSDIAEILQ KITDSEDVAV IIEGEHGCMT TRGIKKPGTK TITTTLRGKF 190 NTDPIVSNKL MMLYTK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000939 Genomic DNA. Translation: ACA46624.1.
RefSeq	YP_001781192.1.
3D structure databases
SMR	B1IL94. Positions 3-193.
ModBase	Search...
Genome annotation databases
GeneID	6150026.
GenomeReviews	Gene locus CLD_2996 in contig CP000939_GR.
KEGG	cbb:CLD_2996.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG370191.
OMA	GVQKQGS.
Family and domain databases
HAMAP	MF_00223. FolE. [Tree]
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. [Graphical view]
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00063. folE. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCH1_CLOBK

Accession

Primary (citable) accession number: B1IL94

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	February 9, 2010
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)