B1IKV7 (B1IKV7_CLOBK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase HAMAP MF_00555
EC=6.3.1.1 HAMAP MF_00555

Alternative name(s):
Asparagine synthetase A HAMAP MF_00555

Gene names

Name:	asnA HAMAP MF_00555 EMBL ACA44762.1
Ordered Locus Names:	CLD_3049

Organism

Clostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]

Taxonomic identifier

498213 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555 SAAS SAAS004618
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555 SAAS SAAS004618
Subcellular location	Cytoplasm By similarity HAMAP MF_00555 SAAS SAAS004618.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily. HAMAP MF_00555

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis HAMAP MF_00555 SAAS SAAS004618
Cellular component	Cytoplasm HAMAP MF_00555 SAAS SAAS004618
Ligand	ATP-binding HAMAP MF_00555 SAAS SAAS004618 Nucleotide-binding
Molecular function	Ligase HAMAP MF_00555 SAAS SAAS004618 EMBL ACA44762.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1IKV7 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: A0F5EAFD6FFC3A47	FASTA	340	39,507
	10 20 30 40 50 60 MIKFKDRLMI PEGYESTLGI RETEVAIKKV KDFFERTLAE KLNITRVSAP LFVRKNTGMN 70 80 90 100 110 120 DNLNGVERPV AFDMKDLQDE MIEIVHSLAK WKRMALHRYD FKVGEGLYTD MNAIRRDEDL 130 140 150 160 170 180 DNLHSIYVDQ WDWEKVIKKE DRNKETLKAI VNKIYSAFKE TERYICSQYE GFNEILPEEI 190 200 210 220 230 240 CFITSQELQD TYPNLDSKER EDAITKEKGA VFLMEIGGVL ASGEKHDGRA PDYDDWTLNG 250 260 270 280 290 300 DILFWNPVLE RAFELSSMGI RVDEEALEKQ LKITGCEDRK NLEFHRLLLE GKLPYTVGGG 310 320 330 340 IGQSRICMYF LRKAHIGEVQ ASIWPDLMIE DCEKAKIKLL

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000939 Genomic DNA. Translation: ACA44762.1.
RefSeq	YP_001781139.1. NC_010516.1.
3D structure databases
ProteinModelPortal	B1IKV7.
SMR	B1IKV7. Positions 21-334.
ModBase	Search...
Protein-protein interaction databases
STRING	B1IKV7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6150010.
GenomeReviews	Gene locus CLD_3049 in contig CP000939_GR.
KEGG	cbb:CLD_3049.
PATRIC	19403498. VBICloBot127283_1653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
ProtClustDB	PRK05425.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1IKV7_CLOBK

Accession

Primary (citable) accession number: B1IKV7

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)