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Protein

16S rRNA (cytosine(967)-C(5))-methyltransferase

Gene

sun

Organism
Clostridium botulinum (strain Okra / Type B1)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.SAAS annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei283S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei310S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei328S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei381NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferasePROSITE-ProRule annotationSAAS annotationImported, RNA-bindingPROSITE-ProRule annotationSAAS annotation, Transferase
Biological processrRNA processingSAAS annotation
LigandS-adenosyl-L-methioninePROSITE-ProRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
16S rRNA (cytosine(967)-C(5))-methyltransferaseSAAS annotation (EC:2.1.1.176SAAS annotation)
Gene namesi
Name:sunImported
Ordered Locus Names:CLD_2130Imported
OrganismiClostridium botulinum (strain Okra / Type B1)Imported
Taxonomic identifieri498213 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000008541 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm SAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliB1IIL8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 442SAM_MT_RSMB_NOPInterPro annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni259 – 265S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000037300
KOiK03500
OMAiLRVNRQH

Family and domain databases

Gene3Di1.10.940.10, 1 hit
InterProiView protein in InterPro
IPR001678 MeTrfase_RsmB/NOP2
IPR035926 NusB-like_sf
IPR006027 NusB_RsmB_TIM44
IPR023267 RCMT
IPR004573 rRNA_ssu_MeTfrase_B
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF01029 NusB, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF48013 SSF48013, 1 hit
SSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00563 rsmB, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

B1IIL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNAREVALD VLKAVLFEGA YSNIVLSKKL NKSNLKDNDK ALITEIVYGT
60 70 80 90 100
LKYKETLDII IQSYLRNPIK TMDKNIVNIL RITIYQIRYL DKIPSFAAVN
110 120 130 140 150
EAVEMSKKIS IKYSKLVNGV LRNYIRTYKN KKFYDHRNNL EKFSFMYSYP
160 170 180 190 200
KWLIKMFISQ YGIEIAEKIL KGLNERPNIT VRVNNLKIDY DEAFEKLGEY
210 220 230 240 250
GYNIEEGYIC PEAIQIIKGK NIEKNPLFIE GNITVQDESA MLVAPSMELD
260 270 280 290 300
EESIVLDLCS APGGKTTHIS EIMNNKSKVY AYDVHQNKLS LIESNAKRLG
310 320 330 340 350
IKNIETDVGD AAVFNKKLKE KAHRVLMDVP CSGLGIIRKK PEIKWTKNER
360 370 380 390 400
EIKNIIAIQR KIMNNGASYL KEGGILLYST CTLNKEENEE NINWFLKKHK
410 420 430 440
NFKIEPLYYG DVDNIIYHKE GFVSILPNDK MDGFFIAKLK KC
Length:442
Mass (Da):50,963
Last modified:April 29, 2008 - v1
Checksum:i1F1475AC7DE1B098
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000939 Genomic DNA Translation: ACA45176.1
RefSeqiWP_004440956.1, NC_010516.1

Genome annotation databases

EnsemblBacteriaiACA45176; ACA45176; CLD_2130
KEGGicbb:CLD_2130

Similar proteinsi

Entry informationi

Entry nameiB1IIL8_CLOBK
AccessioniPrimary (citable) accession number: B1IIL8
Entry historyiIntegrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health