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B1II43

- PNP_CLOBK

UniProt

B1II43 - PNP_CLOBK

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, CLD_2228
Organism
Clostridium botulinum (strain Okra / Type B1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi485 – 4851Magnesium By similarity
Metal bindingi491 – 4911Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCBOT498213:GCNI-2381-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:CLD_2228
OrganismiClostridium botulinum (strain Okra / Type B1)
Taxonomic identifieri498213 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000008541: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 702702Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_1000192473Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi498213.CLD_2228.

Structurei

3D structure databases

ProteinModelPortaliB1II43.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini552 – 61261KHAdd
BLAST
Domaini622 – 69069S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiISMEDTH.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1II43-1 [UniParc]FASTAAdd to Basket

« Hide

MIHTLETTVA GRKMKVDFGK TGMLSNAAIF MSYGDTVVMI NANASKEPRE    50
GIDFFPLSVD YEERLYSVGK IPGGFIKREG KPSDKSILHA RSIDRPLRPL 100
FPKGYRNDVQ IVNTVLSVEQ DNLPEILAIN GSSLALCLSS IPFTTPVAAV 150
SVGLVDGEFI INPTVAQREN TILDLTVCAT KERVMMVEAG GQEIDEETMY 200
SAIIFGFEEC KNIVAFQEEA VAKFGKTKNE PVLYKADEEV EKEVKSFAFD 250
MIKEAMYIMD KDERNAQLDK VKEKISEEFS EKYEDKGADI AEVIYKTQKE 300
IVRNMLLNED RRPDGRAFDE VRPISCEVGI LPRTHGTGLF TRGLTQVMTV 350
ATLGALGDVQ ILDGIAEEES KRYMHHYNFP SYSVGEVRPL RGPGRREIGH 400
GALAERALEP LIPSQEEFPY TIRLVSEVLS SNGSTSQASV CGSTLALLDA 450
GVPIKRPAAG IAMGLITSED LEKEKVITDI QGIEDFFGDM DFKVAGTEKG 500
ITSIQFDTKI KGLSNSCVKD ALEGAKKARL HILGKIKECI PEPRKELSKY 550
APRTEIICID PEKIRDVIGA GGKVINKIIA DTNVKIEIKE DGKIFVTSNN 600
EPEGVKKAIS IIEGLTKEVV QGEIYLGKVT KTTNFGAFVE ILPGKEGLVH 650
ISKLDFARVE KVEDVVSVGD EILVKVTDID NQGRINLSRK DAIAKKEEEK 700
DK 702
Length:702
Mass (Da):77,422
Last modified:April 29, 2008 - v1
Checksum:i36681E024BCEF50A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000939 Genomic DNA. Translation: ACA45744.1.
RefSeqiWP_015957944.1. NC_010516.1.
YP_001781955.1. NC_010516.1.

Genome annotation databases

EnsemblBacteriaiACA45744; ACA45744; CLD_2228.
GeneIDi6148451.
KEGGicbb:CLD_2228.
PATRICi19405078. VBICloBot127283_2443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000939 Genomic DNA. Translation: ACA45744.1 .
RefSeqi WP_015957944.1. NC_010516.1.
YP_001781955.1. NC_010516.1.

3D structure databases

ProteinModelPortali B1II43.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 498213.CLD_2228.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA45744 ; ACA45744 ; CLD_2228 .
GeneIDi 6148451.
KEGGi cbb:CLD_2228.
PATRICi 19405078. VBICloBot127283_2443.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi ISMEDTH.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CBOT498213:GCNI-2381-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
    Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
    PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Okra / Type B1.

Entry informationi

Entry nameiPNP_CLOBK
AccessioniPrimary (citable) accession number: B1II43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: September 3, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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