B1IH18 (MURI_CLOBK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate racemase
EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	CLD_0921

Organism

Clostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]

Taxonomic identifier

498213 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Glutamate racemase HAMAP MF_00258		PRO_1000114038

Sequences

	Sequence		Length	Mass (Da)
	B1IH18 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 0AB84BDD97583B2A	FASTA	257	28,848
	10 20 30 40 50 60 MSINDKPIGF FDSGVGGISV LKEAFKLLPK EDFLYYGDSK NAPYGTKKVE EVKALTFNAT 70 80 90 100 110 120 DFLMNKGIKA LVVACNTATS VTINDLRENY DIPIIGIEPA LKPAVELKKG GKIIIMATPM 130 140 150 160 170 180 TLAEKKFANL MDLYKETEDI EPLPCPGLPE LIEQGIVSGD VIYNYLKDKF SKYDNEKISS 190 200 210 220 230 240 IVLGCTHYPF IEKTLKEVTY NKACIIDGSF GTSRELKRQL KNSNMLREEN RVGKVTIFNS 250 REDKDIIDLS YKLFNMK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Okra / Type B1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000939 Genomic DNA. Translation: ACA44853.1.
RefSeq	YP_001783201.1. NC_010516.1.
3D structure databases
ProteinModelPortal	B1IH18.
ModBase	Search...
Protein-protein interaction databases
STRING	B1IH18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6149015.
GenomeReviews	Gene locus CLD_0921 in contig CP000939_GR.
KEGG	cbb:CLD_0921.
PATRIC	19407656. VBICloBot127283_3679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG645102.
OMA	QCHVRLV.
ProtClustDB	PRK00865.
Enzyme and pathway databases
BioCyc	CBOT498213:CLD_0921-MONOMER.
Family and domain databases
HAMAP	MF_00258. Glu_racemase. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
KO	K01776.
PANTHER	PTHR21198. PTHR21198. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
SUPFAM	SSF53681. Asp/Glu_race. 2 hits.
TIGRFAMs	TIGR00067. Glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_CLOBK

Accession

Primary (citable) accession number: B1IH18

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program