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Protein

GMP synthase [glutamine-hydrolyzing]

Gene

guaA

Organism
Clostridium botulinum (strain Okra / Type B1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of GMP from XMP.UniRule annotation

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.UniRule annotation

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] (guaA)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei82NucleophileUniRule annotation1
Active sitei169UniRule annotation1
Active sitei171UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi223 – 229ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

GMP biosynthesis, Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Protein family/group databases

MEROPSiC26.957.

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing]UniRule annotation (EC:6.3.5.2UniRule annotation)
Alternative name(s):
GMP synthetaseUniRule annotation
Glutamine amidotransferaseUniRule annotation
Gene namesi
Name:guaAUniRule annotation
Ordered Locus Names:CLD_1228
OrganismiClostridium botulinum (strain Okra / Type B1)
Taxonomic identifieri498213 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000008541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001202591 – 510GMP synthase [glutamine-hydrolyzing]Add BLAST510

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1IFD1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 195Glutamine amidotransferase type-1UniRule annotationAdd BLAST191
Domaini196 – 385GMPS ATP-PPaseUniRule annotationAdd BLAST190

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation
Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000223964.
KOiK01951.
OMAiMILVLDF.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1IFD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKELVLVVD FGGQYNQLIA RRVRENRVYC EIVPYTTSIE DIKEKAPKGI
60 70 80 90 100
IFTGGPNSVY GENAPRVQKE LFDLGIPVLG ICYGDQLMAH SLEGEVTSPE
110 120 130 140 150
KREYGKTDVN LDNSSLLFKD MKEKDQCWMS HTDYISKVPK GFKIIATTDE
160 170 180 190 200
CPCAAMENAE KKLYGVQFHP EVEHTLFGKK MLKNFLFNVC NLKGDWSMSS
210 220 230 240 250
FAEQQIKAIK EKVGDKKVIC ALSGGVDSSV AAVIVHKAIG KQLTCIFVDH
260 270 280 290 300
GLLRKDEGDQ VEKIFKDQFD MNLIRVNAQD RFLGKLKGVS DPERKRKIIG
310 320 330 340 350
EEFIRVFEEE AKKLGDISFL VQGTIYPDIV ESGTNTSATI KSHHNVGGLP
360 370 380 390 400
EDMEFKLIEP LRELFKDEVR AVGEELGIPH KLVWRQPFPG PGLAIRVLGE
410 420 430 440 450
VTEEKLAITR EADAIFREEI AKAGLEEKIW QYFACLPNIQ SVGVMGDERT
460 470 480 490 500
YCHTIALRAV TSSDAMTSDW ARIPYEVLDK VSRRIVNEVK EVNRIVYDVT
510
SKPPATIEWE
Length:510
Mass (Da):57,499
Last modified:April 29, 2008 - v1
Checksum:i9EEB9595BA30DDCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000939 Genomic DNA. Translation: ACA45861.1.
RefSeqiWP_003400685.1. NC_010516.1.

Genome annotation databases

EnsemblBacteriaiACA45861; ACA45861; CLD_1228.
KEGGicbb:CLD_1228.
PATRICi19407050. VBICloBot127283_3399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000939 Genomic DNA. Translation: ACA45861.1.
RefSeqiWP_003400685.1. NC_010516.1.

3D structure databases

ProteinModelPortaliB1IFD1.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC26.957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA45861; ACA45861; CLD_1228.
KEGGicbb:CLD_1228.
PATRICi19407050. VBICloBot127283_3399.

Phylogenomic databases

HOGENOMiHOG000223964.
KOiK01951.
OMAiMILVLDF.

Enzyme and pathway databases

UniPathwayiUPA00189; UER00296.

Family and domain databases

CDDicd01742. GATase1_GMP_Synthase. 1 hit.
cd01997. GMP_synthase_C. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.40.50.880. 1 hit.
HAMAPiMF_00344. GMP_synthase. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR001674. GMP_synth_C.
IPR004739. GMP_synth_GATase.
IPR022955. GMP_synthase.
IPR025777. GMPS_ATP_PPase_dom.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00958. GMP_synt_C. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00884. guaA_Cterm. 1 hit.
TIGR00888. guaA_Nterm. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS51553. GMPS_ATP_PPASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUAA_CLOBK
AccessioniPrimary (citable) accession number: B1IFD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.