Reviewed, UniProtKB/Swiss-Prot B1IEV3 (GCST_CLOBK)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	CLD_0070

Organism

Clostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]

Taxonomic identifier

498213 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Aminomethyltransferase HAMAP MF_00259		PRO_1000114089

Sequences

	Sequence		Length	Mass (Da)
	B1IEV3-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 88802A4E34D06960	FASTA	370	41,723
	10 20 30 40 50 60 MEDLKVTPLR GVYEEYGGKI VDFAGYELPT QFKGFLHEHH TVREKAGLFD VSHMGEVMVT 70 80 90 100 110 120 GKDAGKFIQY LMTNDINILK DNEVLYTFMC NEDGGVIDDL LVYKFAENEF FLVINASNKD 130 140 150 160 170 180 KDVKWIMDHK GDFDVEIVDE SDSIAQLALQ GPLAEEILQK IVDIDLQEIK FFKLKRDVLV 190 200 210 220 230 240 NGKKCLVSRT GYTGEDGFEI YCKPEDAKGL WHAILNAGKE EGVQPIGLGA RDTLRFEASL 250 260 270 280 290 300 LLYGNEMDET ITPLEVGMGF FVKLKVEEDF IGKDALIKQK AEGVTRKLVG FELLDKGIPR 310 320 330 340 350 360 HGYEVIKDGK VIGHVTTGYK SPTLNKAIGL ALVEEQYSKI GTEFNIKVRK KELKAVAIDK 370 RFYTKKTKTK

References

[1]

"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids." Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S. PLoS ONE 2:E1271-E1271(2007) [PubMed: 18060065] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000939 Genomic DNA. Translation: ACA43829.1.
RefSeq	YP_001780325.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6150849.
GenomeReviews	Gene locus CLD_0070 in contig CP000939_GR.
KEGG	cbb:CLD_0070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LMTNDIN.
Family and domain databases
HAMAP	MF_00259. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_CLOBK

Accession

Primary (citable) accession number: B1IEV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)