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B1IE18 (KITH_CLOBK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:tdk
Ordered Locus Names:CLD_0650
OrganismClostridium botulinum (strain Okra / Type B1) [Complete proteome] [HAMAP]
Taxonomic identifier498213 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length191 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00124

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00124.

Sequence similarities

Belongs to the thymidine kinase family.

Ontologies

Keywords
   Biological processDNA synthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thymidine kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 191191Thymidine kinase HAMAP-Rule MF_00124
PRO_1000095429

Regions

Nucleotide binding15 – 228ATP By similarity
Nucleotide binding88 – 914ATP By similarity

Sites

Active site891Proton acceptor Potential
Metal binding1451Zinc By similarity
Metal binding1481Zinc By similarity
Metal binding1831Zinc By similarity
Metal binding1861Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
B1IE18 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 524B3EA8664BD59E

FASTA19121,545
        10         20         30         40         50         60 
MYGPKDHGWI EVVAGPMYSG KTEELIRRIR RAEIAKQKVQ VFKPEIDNRY SKQDVVSHAG 

        70         80         90        100        110        120 
DKIQSVPVKS SKEILEKLLD DTDVIGIDEA QFFDDFLVEI VSKIANNNRR VICAGLDMDF 

       130        140        150        160        170        180 
KGEPFGPMPK LMAIAEFVDK IQAVCMVCNN PATRTQRLIN GKPAKKSDPV VLIGAQESYE 

       190 
ARCRKCHRVP R 

« Hide

References

[1]"Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4 and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within plasmids."
Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C., Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.
PLoS ONE 2:E1271-E1271(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Okra / Type B1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000939 Genomic DNA. Translation: ACA45010.1.
RefSeqYP_001779763.1. NC_010516.1.

3D structure databases

ProteinModelPortalB1IE18.
SMRB1IE18. Positions 2-191.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING498213.CLD_0650.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA45010; ACA45010; CLD_0650.
GeneID6150339.
KEGGcbb:CLD_0650.
PATRIC19400755. VBICloBot127283_0295.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1435.
HOGENOMHOG000076390.
KOK00857.
OMAKVHAICV.
OrthoDBEOG69D3J2.
ProtClustDBPRK04296.

Enzyme and pathway databases

BioCycCBOT498213:GCNI-170-MONOMER.

Family and domain databases

HAMAPMF_00124. Thymidine_kinase.
InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKITH_CLOBK
AccessionPrimary (citable) accession number: B1IE18
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families