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Protein

UDP-N-acetylglucosamine 1-carboxyvinyltransferase

Gene

murA1

Organism
Streptococcus pneumoniae (strain Hungary19A-6)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine (By similarity). Target for the antibiotic fosfomycin.UniRule annotation1 Publication

Catalytic activityi

Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921UDP-N-acetylglucosamineBy similarity
Active sitei116 – 1161Proton donorUniRule annotation
Binding sitei306 – 3061UDP-N-acetylglucosamineBy similarity
Binding sitei328 – 3281UDP-N-acetylglucosamine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Pyruvate

Enzyme and pathway databases

BioCyciSPNE487214:GHY0-1152-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.7UniRule annotation)
Alternative name(s):
Enoylpyruvate transferaseUniRule annotation
Short name:
EPTCurated
UDP-N-acetylglucosamine enolpyruvyl transferaseUniRule annotation
Gene namesi
Name:murA1Imported
Synonyms:murAUniRule annotation
Ordered Locus Names:SPH_1173Imported
OrganismiStreptococcus pneumoniae (strain Hungary19A-6)Imported
Taxonomic identifieri487214 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002163 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419UDP-N-acetylglucosamine 1-carboxyvinyltransferasePRO_0000436447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei116 – 11612-(S-cysteinyl)pyruvic acid O-phosphothioketalUniRule annotation

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZH4X-ray1.80A1-419[»]
ProteinModelPortaliB1IBM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232Phosphoenolpyruvate bindingBy similarity
Regioni121 – 1255UDP-N-acetylglucosamine bindingBy similarity

Sequence similaritiesi

Belongs to the EPSP synthase family. MurA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000075602.
KOiK00790.
OMAiRGYDRIQ.
OrthoDBiEOG68M4GK.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00111. MurA.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
IPR005750. UDP_GlcNAc_COvinyl_MurA.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01072. murA. 1 hit.

Sequencei

Sequence statusi: Complete.

B1IBM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIVINGGL PLQGEITISG AKNSVVALIP AIILADDVVT LDCVPDISDV
60 70 80 90 100
ASLVEIMELM GATVKRYDDV LEIDPRGVQN IPMPYGKINS LRASYYFYGS
110 120 130 140 150
LLGRFGEATV GLPGGCDLGP RPIDLHLKAF EAMGATASYE GDNMKLSAKD
160 170 180 190 200
TGLHGASIYM DTVSVGATIN TMIAAVKANG RTIIENAARE PEIIDVATLL
210 220 230 240 250
NNMGAHIRGA GTNIIIIDGV ERLHGTRHQV IPDRIEAGTY ISLAAAVGKG
260 270 280 290 300
IRINNVLYEH LEGFIAKLEE MGVRMTVSED SIFVEEQSNL KAINIKTAPY
310 320 330 340 350
PGFATDLQQP LTPLLLRANG RGTIVDTIYE KRVNHVFELA KMDADISTTN
360 370 380 390 400
GHILYTGGRD LRGTSVKATD LRAGAALVIA GLMAEGKTEI TNIEFILRGY
410
SDIIEKLRNL GADIRLVED
Length:419
Mass (Da):45,055
Last modified:April 29, 2008 - v1
Checksum:iA217CD0CB16DF8F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000936 Genomic DNA. Translation: ACA37082.1.
RefSeqiWP_001227085.1. NC_010380.1.

Genome annotation databases

EnsemblBacteriaiACA37082; ACA37082; SPH_1173.
KEGGispv:SPH_1173.
PATRICi19692457. VBIStrPne34925_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000936 Genomic DNA. Translation: ACA37082.1.
RefSeqiWP_001227085.1. NC_010380.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZH4X-ray1.80A1-419[»]
ProteinModelPortaliB1IBM3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA37082; ACA37082; SPH_1173.
KEGGispv:SPH_1173.
PATRICi19692457. VBIStrPne34925_1180.

Phylogenomic databases

HOGENOMiHOG000075602.
KOiK00790.
OMAiRGYDRIQ.
OrthoDBiEOG68M4GK.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciSPNE487214:GHY0-1152-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00111. MurA.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
IPR005750. UDP_GlcNAc_COvinyl_MurA.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01072. murA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of Streptococcus pneumoniae strain Hungary 19A-6."
    Hotopp J.D., Censini S., Masignani V., Covacci A., Tettelin H.
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hungary19A-6Imported.
  2. "Heteroresistance to fosfomycin is predominant in Streptococcus pneumoniae and depends on the murA1 gene."
    Engel H., Gutierrez-Fernandez J., Fluckiger C., Martinez-Ripoll M., Muhlemann K., Hermoso J.A., Hilty M., Hathaway L.J.
    Antimicrob. Agents Chemother. 57:2801-2808(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), FUNCTION, MISCELLANEOUS.
    Strain: Hungary19A-61 Publication.

Entry informationi

Entry nameiMURA1_STRPI
AccessioniPrimary (citable) accession number: B1IBM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: April 29, 2008
Last modified: July 6, 2016
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This strain of S.pneumoniae has no heteroresistance to fosfomycin unlike other strains of this bacterium in which MurA1 is required for heteroresistance along with other yet unknown factor(s). Heteroresistance is the ability of a clonal population to grow one or several subpopulations at a frequency of 10(-7) to 10(-3) in the presence of a higher antibiotic concentration than that predicted to be effective my measurement of the minimum inhibitory concentration (MIC).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.