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B1I8Y2 (SYL_STRPI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SPH_0372
OrganismStreptococcus pneumoniae (strain Hungary19A-6) [Complete proteome] [HAMAP]
Taxonomic identifier487214 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length833 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 833833Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091370

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6131ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1I8Y2 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 07FABC51B8B69BA2

FASTA83394,384
        10         20         30         40         50         60 
MSFYNHKEIE PKWQGYWAEH HTFKTGTDAS KPKFYALDMF PYPSGAGLHV GHPEGYTATD 

        70         80         90        100        110        120 
ILSRYKRAQG YNVLHPMGWD AFGLPAEQYA MDTGNDPAEF TAENIANFKR QINALGFSYD 

       130        140        150        160        170        180 
WDREVNTTDP NYYKWTQWIF TKLYEKGLAY EAEVPVNWVE ELGTAIANEE VLPDGTSERG 

       190        200        210        220        230        240 
GYPVVRKPMR QWMLKITAYA ERLLNDLDEL DWPESIKDMQ RNWIGKSTGA NVTFKVKGTD 

       250        260        270        280        290        300 
KEFTVFTTRP DTLFGATFTV LAPEHELVDA ITSTEQAEAV ADYKHQASLK SDLARTDLAK 

       310        320        330        340        350        360 
EKTGVWTGAY AINPVNGKEI PIWIADYVLA SYGTGAVMAV PAHDQRDWEF AKQFDLPIVE 

       370        380        390        400        410        420 
VLEGGNVEEA AYTEDGLHVN SDFLDGLNKE DAIAKIVAWL EEKGCGQEKV TYRLRDWLFS 

       430        440        450        460        470        480 
RQRYWGEPIP IIHWEDGTST AVPESELPLV LPVTKDIRPS GTGESPLANL TDWLEVTRED 

       490        500        510        520        530        540 
GVKGRRETNT MPQWAGSSWY YLRYIDPHNT EKLADEDLLK QWLPVDIYVG GAEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKFLY DLGVVPTKEP FQKLFNQGMI LGTSYRDHRG ALVATDKVEK RDGSFFHVET 

       610        620        630        640        650        660 
GEELEQAPAK MSKSLKNVVN PDDVVEQYGA DTLRVYEMFM GPLDASIAWS EEGLEGSRKF 

       670        680        690        700        710        720 
LDRVYRLITS KEILAENNGA LDKVYNETVK AVTEQIESLK FNTAIAQLMV FVNAANKEDK 

       730        740        750        760        770        780 
LYVDYAKGFI QLIAPFAPHL AEELWQTVAE TGESISYVAW PTWDESKLVE DEIEIVVQIK 

       790        800        810        820        830 
GKVRAKLMVA KDLSREELQE IALADEKVKA EIDGKEIVKV IAVPNKLVNI VVK 

« Hide

References

[1]"Complete sequence of Streptococcus pneumoniae strain Hungary 19A-6."
Hotopp J.D., Censini S., Masignani V., Covacci A., Tettelin H.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hungary19A-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000936 Genomic DNA. Translation: ACA37591.1.
RefSeqYP_001693783.1. NC_010380.1.

3D structure databases

ProteinModelPortalB1I8Y2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING487214.SPH_0372.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA37591; ACA37591; SPH_0372.
GeneID6028274.
KEGGspv:SPH_0372.
PATRIC19690828. VBIStrPne34925_0368.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMADKPKYYA.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSPNE487214:GHY0-365-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STRPI
AccessionPrimary (citable) accession number: B1I8Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: June 11, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries