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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Desulforudis audaxviator (strain MP104C)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-1912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Daud_1865
OrganismiDesulforudis audaxviator (strain MP104C)
Taxonomic identifieri477974 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis
ProteomesiUP000008544 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Leucine--tRNA ligasePRO_1000091313Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi477974.Daud_1865.

Structurei

3D structure databases

ProteinModelPortaliB1I5R7.
SMRiB1I5R7. Positions 3-761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi581 – 5855"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1I5R7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKYVFAEI EEKLRRRWET EGIYHVPDFS NRPKYYCLEM FPYPSGNLHM
60 70 80 90 100
GHVRNYAIGD VVARFKTMRG YDVLHPMGWD AFGLPAENAA IQHGVPPARW
110 120 130 140 150
TWDNINVMRT QLKLLGVSYD WRRELATCHP GYYRWTQWLF LQFYHRGLAY
160 170 180 190 200
KAKAPVNWCP SCATVLANEQ VVAGGCERCK TAVERRELEQ WFFRITAYAD
210 220 230 240 250
RLLKDLAKLP GWPEKVKVMQ ENWIGRSTGA EIAFPLAGTD EAIRVFTTRP
260 270 280 290 300
DTLGGVTYMT IAPEHPLVSR VTTPEHRAAV EAFTERARSL SELDRTAGEH
310 320 330 340 350
EKEGLFTGAY CVNPLTGEQV PVFVANYVLM EYGTGCVMGV PAHDQRDFEF
360 370 380 390 400
ARKYGLPVRV VIQPEGDLLD GDTMSQAYTG PGRLVNTPGF DRMANAEAIP
410 420 430 440 450
AITRYLEARG AARFQVQYRL RDWLISRQRY WGAPIPIVYC EGCGVVPVPE
460 470 480 490 500
EGLPVLLPED VAFKPTGRSP LTESPDFVNT TCPTCGGPAR RETDTMDTFM
510 520 530 540 550
CSSWYYFRFT SPREENGPWG LERVDRWLPV DQYIGGVEHA ILHLMYSRFF
560 570 580 590 600
TKVLYDMGLV KVQEPFTNLL TQGMVLKDGA KMSKSKGNVV SPEDILNRYG
610 620 630 640 650
ADTTRLFVLF AAPPERDLEW SDQGVEGCYR FLQRVWRLVN SVADEIRGAA
660 670 680 690 700
PVPSANLVGV NRSMRRLTHQ TIKKVTEDIE TRFNFNTAIS AAMELVNGMY
710 720 730 740 750
HFRDRVAPVN RDPAVMREAV ERLLLLLAPF APFLADELWA RTGHPESIHR
760 770 780 790 800
EPWPEYDPEL LVEDQVEIVV QINGRVRDRL MVAADIAPEA MRDTVLEQPR
810 820
VQALVAGKEI VKVVPVPGKL VNIVVR
Length:826
Mass (Da):93,865
Last modified:April 29, 2008 - v1
Checksum:i6363D3EDDC6C95AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA60358.1.
RefSeqiWP_012302934.1. NC_010424.1.
YP_001717990.1. NC_010424.1.

Genome annotation databases

EnsemblBacteriaiACA60358; ACA60358; Daud_1865.
KEGGidau:Daud_1865.
PATRICi31970606. VBICanDes92303_1923.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA60358.1.
RefSeqiWP_012302934.1. NC_010424.1.
YP_001717990.1. NC_010424.1.

3D structure databases

ProteinModelPortaliB1I5R7.
SMRiB1I5R7. Positions 3-761.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi477974.Daud_1865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA60358; ACA60358; Daud_1865.
KEGGidau:Daud_1865.
PATRICi31970606. VBICanDes92303_1923.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-1912-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Desulforudis audaxviator MP104C."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MP104C.

Entry informationi

Entry nameiSYL_DESAP
AccessioniPrimary (citable) accession number: B1I5R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 1, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.