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B1I4Z1 (SYE_DESAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:Daud_1531
OrganismDesulforudis audaxviator (strain MP104C) [Complete proteome] [HAMAP]
Taxonomic identifier477974 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000367664

Regions

Motif10 – 2011"HIGH" region HAMAP-Rule MF_00022
Motif251 – 2555"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2541ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1I4Z1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 51E4668E23DC1377

FASTA50155,843
        10         20         30         40         50         60 
MDTVRVRFAP SPTGSLHIGG ARTALFNWLF ARHHGGAFIL RLEDTDTGRN IDEAAAQIVS 

        70         80         90        100        110        120 
SLRWLGIDWD EGYDRGGPFG PYRQSERFEL YREEARRLLA NGDAYWCYCT PEDLAAQREE 

       130        140        150        160        170        180 
ARQRGEVPRY DGRCRQLTDD ARREKEAAGI RPALRVKMPK TGTTVVKDRI RGEIGFDNAT 

       190        200        210        220        230        240 
LDDIIVMKSN GGPTYNFACV VDDGAMRISH VIRAEEHLSN TPKQIVLFNL LGYALPEFVH 

       250        260        270        280        290        300 
VPMILAPDRS KLSKRHGATA VDEFRADGFL PEALINYLAL LGWSPGSEQE QFTVEELVAS 

       310        320        330        340        350        360 
FSLDAVSKHA AIYDVKKLTW LNAQYLNSLP QARVVEAVRP FMQEAGYLSA TPNPAELDYL 

       370        380        390        400        410        420 
SRVVEAVRSR VHTLAELRDA SAYFYRSDFE YDDKGVRKHF TKPGVTNILA RGRDALSRLP 

       430        440        450        460        470        480 
AGRFTVEGTE EAFRQVIEEL GVSGGTLIHP TRLALSGRTV GPGLFDIIAV LGKEECLLRL 

       490        500 
DRAIAWIGAN VNNANRTDAC S 

« Hide

References

[1]"Complete sequence of chromosome of Desulforudis audaxviator MP104C."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MP104C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000860 Genomic DNA. Translation: ACA60034.1.
RefSeqYP_001717666.1. NC_010424.1.

3D structure databases

ProteinModelPortalB1I4Z1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING477974.Daud_1531.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA60034; ACA60034; Daud_1531.
GeneID6027551.
KEGGdau:Daud_1531.
PATRIC31969928. VBICanDes92303_1586.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAAMGWEVP.
OrthoDBEOG6DRPF7.
ProtClustDBPRK01406.

Enzyme and pathway databases

BioCycDAUD477974:GH0B-1575-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_DESAP
AccessionPrimary (citable) accession number: B1I4Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries