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B1I4A3

- SYI_DESAP

UniProt

B1I4A3 - SYI_DESAP

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulforudis audaxviator (strain MP104C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-1443-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Daud_1408
OrganismiDesulforudis audaxviator (strain MP104C)
Taxonomic identifieri477974 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis
ProteomesiUP000008544: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000189149Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi477974.Daud_1408.

Structurei

3D structure databases

ProteinModelPortaliB1I4A3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGRRSCQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1I4A3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYSKTLNLP TTEFPMRANL PQREPEIGRF WDEIEVYQLV QAKNAGRPKF
60 70 80 90 100
ILHDGPPYAN GHIHLGTSLN KILKDIVVKF KSMDGYNAPY VPGWDTHGLP
110 120 130 140 150
IEQQAIKQLK IKRSALSPVE FRRMCREYAL KFVDIQRAEF KRLGVRGEWH
160 170 180 190 200
NPYLTLQPHF ETRQIEVFGE MAKRGYIYKG LKSVYWCADC ETALAEAEVE
210 220 230 240 250
YAEKESPSIH VAFPVVNGRG LLPDQDAAIV IWTTTPWTIP SNVAICVHPE
260 270 280 290 300
YAYVLLRSGG RAYLVARDLA GNFRELLGDP GAGVEREYRG EELEGVVCRH
310 320 330 340 350
PFVERDSVVV LADYVTLEQG TGCVHIAPGH GEEDFALGQR YNLPVISPIN
360 370 380 390 400
GKGYFTAEAG NLDGIFYLDA NPVVVRELEA RGALVGYSRM RHQYPHCWRC
410 420 430 440 450
KHPVFYRATE QWFASIDGFR RHLLEAIDRV QWIPGWGRDR IRGMVAGRGD
460 470 480 490 500
WCISRQRVWG VPIPIFYCGD CGRAVITDET ISHLKGLFAE HGSDVWYAWE
510 520 530 540 550
AAELVPPGLV CPHCRGRGDF TKELDTMDVW FDSGSSHWAV LTQPDYWPDL
560 570 580 590 600
QWPADMYLEG SDQHRGWFNS SLTTAVAVTG EPPYRAVLTH GFVVDEQGRK
610 620 630 640 650
MSKSLGNVIE PMEVLKELGA DILRLWVCSA DYRGDLAVSP GILKQMSEAY
660 670 680 690 700
RKIRNTFRFL LGNLQDFDPD RDTVAYTELT ELDRYALLKL HRVTDRVLRA
710 720 730 740 750
YREYEFHQVY HTLYNYCVTD LSAFYLNVIK DRLYCEPARS VNRRGAQTVL
760 770 780 790 800
YAVLDSLVRL LVPVLAYTTE EVWGHFPANG KKPPSVQLLE MPEPNPEYLD
810 820 830 840 850
DELERRWERL LAVRQDVLRA LEAARQEKLI RDALEAEAVL YAEPELLEFL
860 870 880 890 900
RENTVQLPVI FVTSSVRVLP AAEAGGEEAV SGTVAGLKVA VRRAPGTKCV
910 920 930
RCWTYGETGV DPEHPEICPR CATVLSGLNL
Length:930
Mass (Da):105,597
Last modified:April 29, 2008 - v1
Checksum:i2140A91F26C4DDFF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000860 Genomic DNA. Translation: ACA59917.1.
RefSeqiYP_001717549.1. NC_010424.1.

Genome annotation databases

EnsemblBacteriaiACA59917; ACA59917; Daud_1408.
GeneIDi6025894.
KEGGidau:Daud_1408.
PATRICi31969654. VBICanDes92303_1458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000860 Genomic DNA. Translation: ACA59917.1 .
RefSeqi YP_001717549.1. NC_010424.1.

3D structure databases

ProteinModelPortali B1I4A3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 477974.Daud_1408.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA59917 ; ACA59917 ; Daud_1408 .
GeneIDi 6025894.
KEGGi dau:Daud_1408.
PATRICi 31969654. VBICanDes92303_1458.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGRRSCQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DAUD477974:GH0B-1443-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Desulforudis audaxviator MP104C."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MP104C.

Entry informationi

Entry nameiSYI_DESAP
AccessioniPrimary (citable) accession number: B1I4A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3