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B1I4A3

- SYI_DESAP

UniProt

B1I4A3 - SYI_DESAP

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulforudis audaxviator (strain MP104C)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
    Binding sitei603 – 6031ATPUniRule annotation
    Metal bindingi899 – 8991ZincUniRule annotation
    Metal bindingi902 – 9021ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDAUD477974:GH0B-1443-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Daud_1408
    OrganismiDesulforudis audaxviator (strain MP104C)
    Taxonomic identifieri477974 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis
    ProteomesiUP000008544: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000189149Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi477974.Daud_1408.

    Structurei

    3D structure databases

    ProteinModelPortaliB1I4A3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGRRSCQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1I4A3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSKTLNLP TTEFPMRANL PQREPEIGRF WDEIEVYQLV QAKNAGRPKF    50
    ILHDGPPYAN GHIHLGTSLN KILKDIVVKF KSMDGYNAPY VPGWDTHGLP 100
    IEQQAIKQLK IKRSALSPVE FRRMCREYAL KFVDIQRAEF KRLGVRGEWH 150
    NPYLTLQPHF ETRQIEVFGE MAKRGYIYKG LKSVYWCADC ETALAEAEVE 200
    YAEKESPSIH VAFPVVNGRG LLPDQDAAIV IWTTTPWTIP SNVAICVHPE 250
    YAYVLLRSGG RAYLVARDLA GNFRELLGDP GAGVEREYRG EELEGVVCRH 300
    PFVERDSVVV LADYVTLEQG TGCVHIAPGH GEEDFALGQR YNLPVISPIN 350
    GKGYFTAEAG NLDGIFYLDA NPVVVRELEA RGALVGYSRM RHQYPHCWRC 400
    KHPVFYRATE QWFASIDGFR RHLLEAIDRV QWIPGWGRDR IRGMVAGRGD 450
    WCISRQRVWG VPIPIFYCGD CGRAVITDET ISHLKGLFAE HGSDVWYAWE 500
    AAELVPPGLV CPHCRGRGDF TKELDTMDVW FDSGSSHWAV LTQPDYWPDL 550
    QWPADMYLEG SDQHRGWFNS SLTTAVAVTG EPPYRAVLTH GFVVDEQGRK 600
    MSKSLGNVIE PMEVLKELGA DILRLWVCSA DYRGDLAVSP GILKQMSEAY 650
    RKIRNTFRFL LGNLQDFDPD RDTVAYTELT ELDRYALLKL HRVTDRVLRA 700
    YREYEFHQVY HTLYNYCVTD LSAFYLNVIK DRLYCEPARS VNRRGAQTVL 750
    YAVLDSLVRL LVPVLAYTTE EVWGHFPANG KKPPSVQLLE MPEPNPEYLD 800
    DELERRWERL LAVRQDVLRA LEAARQEKLI RDALEAEAVL YAEPELLEFL 850
    RENTVQLPVI FVTSSVRVLP AAEAGGEEAV SGTVAGLKVA VRRAPGTKCV 900
    RCWTYGETGV DPEHPEICPR CATVLSGLNL 930
    Length:930
    Mass (Da):105,597
    Last modified:April 29, 2008 - v1
    Checksum:i2140A91F26C4DDFF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000860 Genomic DNA. Translation: ACA59917.1.
    RefSeqiYP_001717549.1. NC_010424.1.

    Genome annotation databases

    EnsemblBacteriaiACA59917; ACA59917; Daud_1408.
    GeneIDi6025894.
    KEGGidau:Daud_1408.
    PATRICi31969654. VBICanDes92303_1458.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000860 Genomic DNA. Translation: ACA59917.1 .
    RefSeqi YP_001717549.1. NC_010424.1.

    3D structure databases

    ProteinModelPortali B1I4A3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 477974.Daud_1408.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACA59917 ; ACA59917 ; Daud_1408 .
    GeneIDi 6025894.
    KEGGi dau:Daud_1408.
    PATRICi 31969654. VBICanDes92303_1458.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGRRSCQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DAUD477974:GH0B-1443-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Desulforudis audaxviator MP104C."
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P.
      Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MP104C.

    Entry informationi

    Entry nameiSYI_DESAP
    AccessioniPrimary (citable) accession number: B1I4A3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3