Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Desulforudis audaxviator (strain MP104C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation
Metal bindingi921 – 9211ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-1443-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Daud_1408
OrganismiDesulforudis audaxviator (strain MP104C)
Taxonomic identifieri477974 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis
ProteomesiUP000008544 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 930930Isoleucine--tRNA ligasePRO_1000189149Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi477974.Daud_1408.

Structurei

3D structure databases

ProteinModelPortaliB1I4A3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1I4A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSKTLNLP TTEFPMRANL PQREPEIGRF WDEIEVYQLV QAKNAGRPKF
60 70 80 90 100
ILHDGPPYAN GHIHLGTSLN KILKDIVVKF KSMDGYNAPY VPGWDTHGLP
110 120 130 140 150
IEQQAIKQLK IKRSALSPVE FRRMCREYAL KFVDIQRAEF KRLGVRGEWH
160 170 180 190 200
NPYLTLQPHF ETRQIEVFGE MAKRGYIYKG LKSVYWCADC ETALAEAEVE
210 220 230 240 250
YAEKESPSIH VAFPVVNGRG LLPDQDAAIV IWTTTPWTIP SNVAICVHPE
260 270 280 290 300
YAYVLLRSGG RAYLVARDLA GNFRELLGDP GAGVEREYRG EELEGVVCRH
310 320 330 340 350
PFVERDSVVV LADYVTLEQG TGCVHIAPGH GEEDFALGQR YNLPVISPIN
360 370 380 390 400
GKGYFTAEAG NLDGIFYLDA NPVVVRELEA RGALVGYSRM RHQYPHCWRC
410 420 430 440 450
KHPVFYRATE QWFASIDGFR RHLLEAIDRV QWIPGWGRDR IRGMVAGRGD
460 470 480 490 500
WCISRQRVWG VPIPIFYCGD CGRAVITDET ISHLKGLFAE HGSDVWYAWE
510 520 530 540 550
AAELVPPGLV CPHCRGRGDF TKELDTMDVW FDSGSSHWAV LTQPDYWPDL
560 570 580 590 600
QWPADMYLEG SDQHRGWFNS SLTTAVAVTG EPPYRAVLTH GFVVDEQGRK
610 620 630 640 650
MSKSLGNVIE PMEVLKELGA DILRLWVCSA DYRGDLAVSP GILKQMSEAY
660 670 680 690 700
RKIRNTFRFL LGNLQDFDPD RDTVAYTELT ELDRYALLKL HRVTDRVLRA
710 720 730 740 750
YREYEFHQVY HTLYNYCVTD LSAFYLNVIK DRLYCEPARS VNRRGAQTVL
760 770 780 790 800
YAVLDSLVRL LVPVLAYTTE EVWGHFPANG KKPPSVQLLE MPEPNPEYLD
810 820 830 840 850
DELERRWERL LAVRQDVLRA LEAARQEKLI RDALEAEAVL YAEPELLEFL
860 870 880 890 900
RENTVQLPVI FVTSSVRVLP AAEAGGEEAV SGTVAGLKVA VRRAPGTKCV
910 920 930
RCWTYGETGV DPEHPEICPR CATVLSGLNL
Length:930
Mass (Da):105,597
Last modified:April 29, 2008 - v1
Checksum:i2140A91F26C4DDFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA59917.1.
RefSeqiWP_012302502.1. NC_010424.1.

Genome annotation databases

EnsemblBacteriaiACA59917; ACA59917; Daud_1408.
KEGGidau:Daud_1408.
PATRICi31969654. VBICanDes92303_1458.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA59917.1.
RefSeqiWP_012302502.1. NC_010424.1.

3D structure databases

ProteinModelPortaliB1I4A3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi477974.Daud_1408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA59917; ACA59917; Daud_1408.
KEGGidau:Daud_1408.
PATRICi31969654. VBICanDes92303_1458.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-1443-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Desulforudis audaxviator MP104C."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MP104C.

Entry informationi

Entry nameiSYI_DESAP
AccessioniPrimary (citable) accession number: B1I4A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.