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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Desulforudis audaxviator (strain MP104C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-619-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Daud_0601
OrganismiDesulforudis audaxviator (strain MP104C)
Taxonomic identifieri477974 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptococcaceaeCandidatus Desulforudis
Proteomesi
  • UP000008544 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346333Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi477974.Daud_0601.

Structurei

3D structure databases

ProteinModelPortaliB1I258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108FW6. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

B1I258-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGGVIVVGA GLAGAEASWQ LVRRGVPVVL YEMRPRKCTP AHKTGDFAEL
60 70 80 90 100
VCSNSLRAEA LTNAVGLLKE EMRRLGSLIM ACADAHRVPA GGALAVDRQL
110 120 130 140 150
FAAAVTERLT SHRLVTVCRE EITTIPTAEL VILATGPLTS DALADELRRL
160 170 180 190 200
TGQEHLYFFD AVAPIVTLES IDQDRVFRSS RYGRGDPAYL NCPMSREEYE
210 220 230 240 250
RFWEALVAAE RATRHTFERE THFEGCLPVE VIAARGRETL LYGPLKPVGL
260 270 280 290 300
VDPRTGERPY AVVQLRQDNR AGTLYNLVGF QTNLKWGEQR RVFSMIPGLE
310 320 330 340 350
QAEFVRYGVM HRNTYINAPV LLSPNLMLKS RPGLFIAGQL SGVEGYVESA
360 370 380 390 400
AAGLVAGLNA ARLYKGLEPL VFPPETAHGA LINYIVTADP ANFQPMNVNF
410 420 430
GLFPPLPGKR VRRRPERNLA HAQRALERLA AWLTEKGEG
Length:439
Mass (Da):48,337
Last modified:April 29, 2008 - v1
Checksum:iC6BFC35D04A980B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA59135.1.
RefSeqiWP_012301723.1. NC_010424.1.

Genome annotation databases

EnsemblBacteriaiACA59135; ACA59135; Daud_0601.
KEGGidau:Daud_0601.
PATRICi31967959. VBICanDes92303_0627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000860 Genomic DNA. Translation: ACA59135.1.
RefSeqiWP_012301723.1. NC_010424.1.

3D structure databases

ProteinModelPortaliB1I258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi477974.Daud_0601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA59135; ACA59135; Daud_0601.
KEGGidau:Daud_0601.
PATRICi31967959. VBICanDes92303_0627.

Phylogenomic databases

eggNOGiENOG4108FW6. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Enzyme and pathway databases

BioCyciDAUD477974:GH0B-619-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_DESAP
AccessioniPrimary (citable) accession number: B1I258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: April 29, 2008
Last modified: September 7, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.