B1I255 (B1I255_DESAP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
DNA topoisomerase I EMBL ACA59134.1
EC=5.99.1.2 EMBL ACA59134.1

Gene names

Ordered Locus Names:

Daud_0600

Organism

Desulforudis audaxviator (strain MP104C) [Complete proteome] [HAMAP] EMBL ACA59134.1

Taxonomic identifier

477974 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Candidatus Desulforudis

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733
Sequence similarities	Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B1I255 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 9EC6CDD5A1474565	FASTA	711	81,085
	10 20 30 40 50 60 MEKTLVIVES PAKARTLGKF LGKKYEIRAS MGHVRDLPRS QFGVDVDDGF KPKYITIRGK 70 80 90 100 110 120 GDAIKELRAA RKKSNQVLLA SDPDREGEAI AWHLQELLQI DPEEPCRVEF NEITREAVTA 130 140 150 160 170 180 AIKNPRKIDP NRVDAQQARR ILDRLVGYNL SPLLWRKVRK GLSAGRVQSV AVHLICARER 190 200 210 220 230 240 EIEAFVSEEY WTLTALLANR ERERFKARLL KKAGEKLTVP NEEAMKGILD DLKEEVYRVA 250 260 270 280 290 300 DVIRKEKKKN PSPPFTTSTL QQEAYRRLNF TTRKTMMVAQ QLYEGLDLGK EGPVGLVTYI 310 320 330 340 350 360 RTDSTRISPG AQDEAREYIA EHYGKEYVPK QKRVYTGKAR AQDAHEAIRP TSVLRTPEAL 370 380 390 400 410 420 RKYLNPDQLR LYELIWSRFL ASQMVSAVLD TVRAEIVAGE YLFRATGSTV KFPGFTRVYT 430 440 450 460 470 480 ELRDEAKNGE EEEEGALPAL EKGEQLKLVR TTPARHFTQP PARYTEATLV RTLEELGIGR 490 500 510 520 530 540 PSTYAPVVET IQRRGYVVRR GKTLHPTQLG LVVIDLLKEH FPDVINYEFT AELEEKLDRI 550 560 570 580 590 600 EEGSMQWEKV LEEFYRPFRR EVAEAEAKID RVRLDEVTDE ECRKCGRKMV IKMGRYGKFL 610 620 630 640 650 660 ACPGFPECRH TRPIFEETGH RCPKCGGRVV VRRTKKSKVF YGCAGYPGCD FVTWDQPTDQ 670 680 690 700 710 KCPDCQSFLV ARGTRGHPLQ RVSGSAGQSR TFACANPECG YKERKAGRGK A

References

[1]

"Complete sequence of chromosome of Desulforudis audaxviator MP104C." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000860 Genomic DNA. Translation: ACA59134.1.
RefSeq	YP_001716766.1. NC_010424.1.
3D structure databases
ProteinModelPortal	B1I255.
ModBase	Search...
Protein-protein interaction databases
STRING	B1I255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6025471.
GenomeReviews	Gene locus Daud_0600 in contig CP000860_GR.
KEGG	dau:Daud_0600.
PATRIC	31967957. VBICanDes92303_0626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	PREIDIK.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1I255_DESAP

Accession

Primary (citable) accession number: B1I255

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)