Reviewed, UniProtKB/Swiss-Prot B1I0S9 (ISPDF_DESAP)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	Daud_0186

Organism

Desulforudis audaxviator (strain MP104C) [Complete proteome] [HAMAP]

Taxonomic identifier

477974 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Peptococcaceae › Candidatus Desulforudis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_1000146271
Regions
	Region	1 – 230	230	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	231 – 394	164	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	237	1	Divalent metal cation By similarity
	Metal binding	239	1	Divalent metal cation By similarity
	Metal binding	271	1	Divalent metal cation By similarity
	Site	16	1	Transition state stabilizer By similarity
	Site	23	1	Transition state stabilizer By similarity
	Site	155	1	Positions MEP for the nucleophilic attack By similarity
	Site	211	1	Positions MEP for the nucleophilic attack By similarity
	Site	263	1	Transition state stabilizer By similarity
	Site	362	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1I0S9-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 55EB49DD50524718	FASTA	394	41,482
	10 20 30 40 50 60 MADTLAIVVA AGRSSRMGEG PKKQYRLLAG RPVLGRTLEV FEHAPAVDGV VLVVAPGEED 70 80 90 100 110 120 WCREEIVTRF GFTKVVAVVP GGEVRRDSVW AGLQALPPCA LVLVHDGVRP FVTARQIAAV 130 140 150 160 170 180 AEAARECGAA TLAVPPKDTV KLGGPPGAPV STLPRENLWL VQTPQAFRFD VLIKAHHLAR 190 200 210 220 230 240 ERGLAATDDT SLAEAAGYDV RMVPGAYTNI KITTPEDLAF AEALLGGGPV LVGFGYDVHR 250 260 270 280 290 300 LVDDRKLILG GVEVPHDRGL LGHSDADVLV HAVMDALLGA AGAGDIGRWF PDDDPTYRGI 310 320 330 340 350 360 SSMDLLVRVA AFLRERGLET SNLDAVVVAE APRLSPFISR MRDNLASVLG VSPAAVNVKA 370 380 390 TTTEGLGFTG SGAGIAAYAV AALRRIVLPG DRVL

References

[1]

"Complete sequence of chromosome of Desulforudis audaxviator MP104C." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Bruce D., Pitluck S., Lowry S.R., Larimer F., Land M.L., Hauser L., Kyrpides N., Ivanova N.N., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000860 Genomic DNA. Translation: ACA58750.1.
RefSeq	YP_001716382.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6027621.
GenomeReviews	Gene locus Daud_0186 in contig CP000860_GR.
KEGG	dau:Daud_0186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IVLIHDA.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. IPR020555. MECDP_synthase_CS. [Graphical view]
Gene3D	G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_DESAP

Accession

Primary (citable) accession number: B1I0S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	April 29, 2008
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)