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B1HXY7 (SYL_LYSSC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bsph_4241
OrganismLysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP]
Taxonomic identifier444177 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091335

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1HXY7 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: BAF32FB558789C0D

FASTA80591,395
        10         20         30         40         50         60 
MSFNHQQIEK KWQQYWADNK TFKTENETEK PKFFALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LSRFKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT AKNIATFKRQ IQELGFSYDW 

       130        140        150        160        170        180 
DREINTTDPA YYKWTQWIFI QLYKKGLAYV DEVAVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVERRPMKQW MLKITAYADR LIDDLEEVDW PESIKDMQRN WIGRSEGAEV TFGIDGTDEN 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYCVLA PEHKLVEQIT TVEQRQAVED YLEKVKMKSD LERTDLAKEK 

       310        320        330        340        350        360 
TGVFTGAYAV NPINGKKVPI WIADYVLVSY GTGAIMAVPA HDERDYEFAS AFSLDIVPVL 

       370        380        390        400        410        420 
EGGDISKEAF TGDGQHINSD FLNGLNKVEG IAKAIEWLEE KGVGEKKISY RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPMI HWEDGTITPV PEEELPLMLP KTDNIRPSGT GESPLANIAE WVNVVDPATG 

       490        500        510        520        530        540 
KKGRRETNTM PQWAGSSWYF LRYIDPTNTE AIADPELLKR WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD LGVVHTKEPF QKLFNQGMIL GEGNEKMSKS KGNVVNPDEI IESHGSDTLR 

       610        620        630        640        650        660 
LYEMFMGPLE ASVAWSTNGL DGARRFLDRI WRLLVNEEDG TISAKIQVSD DKTLEKSYHQ 

       670        680        690        700        710        720 
TVKKVTEDYE GIRFNTAISQ MMVFINDCYK AEVIPTAYAE GFVKMLAPIA PHIAEELWNL 

       730        740        750        760        770        780 
LGHEGTLSYE QWPTYDESKL VDDEVEVAVQ VAGKVRAKIV VAKDASKEDI EKVALADEKV 

       790        800 
QEYMAGKDLV KVIVIPGKLV NIVVK 

« Hide

References

[1]"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C3-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000817 Genomic DNA. Translation: ACA41702.1.
RefSeqYP_001699832.1. NC_010382.1.

3D structure databases

ProteinModelPortalB1HXY7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING444177.Bsph_4241.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA41702; ACA41702; Bsph_4241.
GeneID6025334.
KEGGlsp:Bsph_4241.
PATRIC22420925. VBILysSph89750_4319.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLSPH444177:GJEL-4211-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LYSSC
AccessionPrimary (citable) accession number: B1HXY7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries