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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Lysinibacillus sphaericus (strain C3-41)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-4211-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Bsph_4241
OrganismiLysinibacillus sphaericus (strain C3-41)
Taxonomic identifieri444177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus
ProteomesiUP000002164 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 805805Leucine--tRNA ligasePRO_1000091335Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi444177.Bsph_4241.

Structurei

3D structure databases

ProteinModelPortaliB1HXY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1HXY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFNHQQIEK KWQQYWADNK TFKTENETEK PKFFALDMFP YPSGAGLHVG
60 70 80 90 100
HPEGYTATDI LSRFKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPAEFT
110 120 130 140 150
AKNIATFKRQ IQELGFSYDW DREINTTDPA YYKWTQWIFI QLYKKGLAYV
160 170 180 190 200
DEVAVNWCPA LGTVLANEEV IDGKSERGGH PVERRPMKQW MLKITAYADR
210 220 230 240 250
LIDDLEEVDW PESIKDMQRN WIGRSEGAEV TFGIDGTDEN FTVFTTRPDT
260 270 280 290 300
LFGATYCVLA PEHKLVEQIT TVEQRQAVED YLEKVKMKSD LERTDLAKEK
310 320 330 340 350
TGVFTGAYAV NPINGKKVPI WIADYVLVSY GTGAIMAVPA HDERDYEFAS
360 370 380 390 400
AFSLDIVPVL EGGDISKEAF TGDGQHINSD FLNGLNKVEG IAKAIEWLEE
410 420 430 440 450
KGVGEKKISY RLRDWLFSRQ RYWGEPIPMI HWEDGTITPV PEEELPLMLP
460 470 480 490 500
KTDNIRPSGT GESPLANIAE WVNVVDPATG KKGRRETNTM PQWAGSSWYF
510 520 530 540 550
LRYIDPTNTE AIADPELLKR WLPVDIYIGG AEHAVLHLLY ARFWHKVLYD
560 570 580 590 600
LGVVHTKEPF QKLFNQGMIL GEGNEKMSKS KGNVVNPDEI IESHGSDTLR
610 620 630 640 650
LYEMFMGPLE ASVAWSTNGL DGARRFLDRI WRLLVNEEDG TISAKIQVSD
660 670 680 690 700
DKTLEKSYHQ TVKKVTEDYE GIRFNTAISQ MMVFINDCYK AEVIPTAYAE
710 720 730 740 750
GFVKMLAPIA PHIAEELWNL LGHEGTLSYE QWPTYDESKL VDDEVEVAVQ
760 770 780 790 800
VAGKVRAKIV VAKDASKEDI EKVALADEKV QEYMAGKDLV KVIVIPGKLV

NIVVK
Length:805
Mass (Da):91,395
Last modified:April 29, 2008 - v1
Checksum:iBAF32FB558789C0D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA41702.1.
RefSeqiWP_012295731.1. NC_010382.1.

Genome annotation databases

EnsemblBacteriaiACA41702; ACA41702; Bsph_4241.
GeneIDi6025334.
KEGGilsp:Bsph_4241.
PATRICi22420925. VBILysSph89750_4319.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA41702.1.
RefSeqiWP_012295731.1. NC_010382.1.

3D structure databases

ProteinModelPortaliB1HXY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444177.Bsph_4241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA41702; ACA41702; Bsph_4241.
GeneIDi6025334.
KEGGilsp:Bsph_4241.
PATRICi22420925. VBILysSph89750_4319.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-4211-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
    Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
    J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C3-41.

Entry informationi

Entry nameiSYL_LYSSC
AccessioniPrimary (citable) accession number: B1HXY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.