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B1HVD0 (B1HVD0_LYSSC) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Valine--tRNA ligase HAMAP-Rule MF_02004
EC=6.1.1.9 HAMAP-Rule MF_02004
Alternative name(s):
Valyl-tRNA synthetase HAMAP-Rule MF_02004
Gene names
Name:valS HAMAP-Rule MF_02004
Ordered Locus Names:Bsph_3964 EMBL ACA41432.1
OrganismLysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP] EMBL ACA41432.1
Taxonomic identifier444177 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Lysinibacillus ›

Protein attributes

Sequence length881 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Catalytic activity

ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val). HAMAP-Rule MF_02004 SAAS SAAS019499

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02004 SAAS SAAS019499

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02004 SAAS SAAS019499.

Domain

The C-terminal coiled-coil domain is crucial for aminoacylation activity By similarity. HAMAP-Rule MF_02004

ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site By similarity. HAMAP-Rule MF_02004

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily. HAMAP-Rule MF_02004

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Coiled coil809 – 88173 By similarity HAMAP-Rule MF_02004
Motif48 – 5811"HIGH" region By similarity HAMAP-Rule MF_02004
Motif525 – 5295"KMSKS" region By similarity HAMAP-Rule MF_02004

Sites

Binding site5281ATP By similarity HAMAP-Rule MF_02004

Sequences

Sequence LengthMass (Da)Tools
B1HVD0 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: A9C830B4BD69BCA6

FASTA881101,173
        10         20         30         40         50         60 
MTENISMPTK YDPQSIEAGR YEWWLQGKFF EAQPESGKKP YSIVIPPPNV TGKLHLGHAW 

        70         80         90        100        110        120 
DTTLQDILIR MKRMQGYDAL WLPGMDHAGI ATQAKVEAKL REDNITRYDL GREKFLEKTW 

       130        140        150        160        170        180 
EWKEEYAGHI RDQWAKLGLG LDYTRERFTL DKGLSEAVNT VFVQLYEKGL IYRGERIINW 

       190        200        210        220        230        240 
DPAAKTALSD IEVIYQDVQG AFYHMKYPLA DGSGYVEVAT TRPETMLGDS GVAVHPKDER 

       250        260        270        280        290        300 
YQHLIGKTVI LPIVGREIPI VADDYVDMEF GTGVVKMTPA HDPNDFEVGN RHNLERILVM 

       310        320        330        340        350        360 
NEDGTMNELA GKYNGMDRFE CRKQIVADLQ EAGVLIRIEE HMHSVGHSER SGAVVEPYLS 

       370        380        390        400        410        420 
AQWFVKMQPL ADSSLELQKD QEGKVNFVPA RFENTYSRWM ENIRDWCISR QLWWGHQIPA 

       430        440        450        460        470        480 
WYHNETGEIY VGKEAPVDAE NWTQDEDVLD TWFSSALWPF STMGWPDEAN EEYKRYYPTN 

       490        500        510        520        530        540 
TLVTGYDIIF FWVSRMIFQG LEFTEQRPFK DVLIHGLVRD GEGRKMSKSL GNGVDPMDVI 

       550        560        570        580        590        600 
EQYGADSLRY FLATGSSPGQ DLRYTTEKVE AVWNFSNKIW NASRFALMNM DGMTYDEIDL 

       610        620        630        640        650        660 
TGEKSVADKW ILTRLNETIE RVTSLAERYE FGEVGRELYN FIWDDFCSWY IEMAKLPLYG 

       670        680        690        700        710        720 
EDEAAKKTTR SILAYVLDQT MRLLHPFMPF ITEEIWQHLP HDGESITVAA WPTVRTDLHF 

       730        740        750        760        770        780 
ADESDNMKLL MDIIRSVRNI RAEVNTPMSK KVPLFISAKD AATVAVLEAN KAYLEKFCNP 

       790        800        810        820        830        840 
DTLTIGEGLE APGQSMTAVI TGAQVFLPLV GLINLEEEIA RLEKELEKWA KEVKLVTGKL 

       850        860        870        880 
SNEKFVSKAP EALVNVEREK LADYESKHAV VLKRLEELKN M

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References

[1]"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C3-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000817 Genomic DNA. Translation: ACA41432.1.
RefSeqYP_001699562.1. NC_010382.1.

3D structure databases

ProteinModelPortalB1HVD0.
ModBaseSearch...

Protein-protein interaction databases

STRING444177.Bsph_3964.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA41432; ACA41432; Bsph_3964.
GeneID6024043.
KEGGlsp:Bsph_3964.
PATRIC22420363. VBILysSph89750_4039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0525.
HOGENOMHOG000020093.
KOK01873.
OMAIMDALIR.

Enzyme and pathway databases

BioCycLSPH444177:GJEL-3937-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02004. Val_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR010978. tRNA-bd_arm.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR019499. Val-tRNA_synth_tRNA-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR002303. Valyl-tRNA_ligase.
[Graphical view]
PANTHERPTHR11946:SF5. PTHR11946:SF5. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF10458. Val_tRNA-synt_C. 1 hit.
[Graphical view]
PRINTSPR00986. TRNASYNTHVAL.
SUPFAMSSF46589. tRNA_binding_arm. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMsTIGR00422. valS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB1HVD0_LYSSC
AccessionPrimary (citable) accession number: B1HVD0
Entry history
Integrated into UniProtKB/TrEMBL: April 29, 2008
Last sequence update: April 29, 2008
Last modified: May 1, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info