B1HU20 (PANC_LYSSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Bsph_1988

Organism

Lysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP]

Taxonomic identifier

444177 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Lysinibacillus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Pantothenate synthetase HAMAP MF_00158		PRO_1000097082
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
	Nucleotide binding	147 – 150	4	ATP By similarity
	Nucleotide binding	184 – 187	4	ATP By similarity
Sites
	Active site	37	1	Proton donor By similarity
	Binding site	61	1	Beta-alanine By similarity
	Binding site	61	1	Pantoate By similarity
	Binding site	153	1	Pantoate By similarity
	Binding site	176	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1HU20 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: EF6C853DEAA3668D	FASTA	284	31,279
	10 20 30 40 50 60 MKVMTTIQAL TAEIQAAKQA HKTIGLVPTM GYLHEGHLTL AQTARAENDL VVMSIFVNPT 70 80 90 100 110 120 QFGPNEDFES YPRDLPRDIA LAESVGVDLV FAPSVEEMYP QDGGIRIHAG EQATILCGAS 130 140 150 160 170 180 RPGHFDGVLQ VVAKLFHLTL PTRAYFGQKD AQQVAIITTM VRDFNFPLDM RVVPIVREED 190 200 210 220 230 240 GLAKSSRNIY LSESEREEAP AIHEALQLAR DSFLATGDVR EALAKAKAHI QIQTHGRIDY 250 260 270 280 IELLAYPDLT PVTETTQQVL LAAAVYIGKT RLIDNSIFTV KEGL

References

[1]

"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species." Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z. J. Bacteriol. 190:2892-2902(2008) [PubMed: 18296527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C3-41.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000817 Genomic DNA. Translation: ACA39575.1.
RefSeq	YP_001697705.1. NC_010382.1.
3D structure databases
ProteinModelPortal	B1HU20.
ModBase	Search...
Protein-protein interaction databases
STRING	B1HU20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6021307.
GenomeReviews	Gene locus Bsph_1988 in contig CP000817_GR.
KEGG	lsp:Bsph_1988.
PATRIC	22416491. VBILysSph89750_2111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	LNMPIQI.
Enzyme and pathway databases
BioCyc	LSPH444177:BSPH_1988-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans-rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit. TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_LYSSC

Accession

Primary (citable) accession number: B1HU20

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program