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B1HTV8 (PUR9_LYSSC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:Bsph_0218
OrganismLysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP]
Taxonomic identifier444177 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000096073

Sequences

Sequence LengthMass (Da)Tools
B1HTV8 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: EEBDCF95D68326D7

FASTA50855,471
        10         20         30         40         50         60 
MTKRALISVS NKDGILEFAK ELVALGYEIL STGGTKKMLQ DNAVAVTAVD EVTKFPEILD 

        70         80         90        100        110        120 
GRVKTLNPMI HGGLLGKFDD DSHQAQMNEH GIEPIEIVCV NLYPFVETIS KPNVTWDDAI 

       130        140        150        160        170        180 
ENIDIGGPTM LRSAAKNHQY VTVIVDSNDY ATVLEELKAG GATTIETRRK LAAKVFRHTA 

       190        200        210        220        230        240 
AYDSYISNYL TEEEFPESLT MTYELKQNLR YGENPHQKAA FYQKRLGSDF SLAYATQLHG 

       250        260        270        280        290        300 
KELSYNNIQD GNAALQIVKE FEMPAAVAVK HMNPCGVGTG VTLEEAFDKA YEADPTSIFG 

       310        320        330        340        350        360 
GIIALNMEVD AATAEKLSHI FLEIIIAPAF SQEALDILTK KKNIRLLTIP FEQAKQDQFN 

       370        380        390        400        410        420 
VVSVEGGLLV QEPDRYGFAN ADIKVVTDRE PTKQEWEALQ LGWSVVKHVK SNAIVVTDSQ 

       430        440        450        460        470        480 
MTLGVGAGQM NRVGAAKIAF EQAGEKAKGA ALASDAFFPM SDTVEAAAAA GITAIIQPGG 

       490        500 
SIKDQDSIDK ANEYGITMIF TGVRHFKH 

« Hide

References

[1]"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C3-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000817 Genomic DNA. Translation: ACA37849.1.
RefSeqYP_001695979.1. NC_010382.1.

3D structure databases

ProteinModelPortalB1HTV8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING444177.Bsph_0218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA37849; ACA37849; Bsph_0218.
GeneID6021866.
KEGGlsp:Bsph_0218.
PATRIC22412892. VBILysSph89750_0365.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMARAFKTDP.
OrthoDBEOG6QCDFF.

Enzyme and pathway databases

BioCycLSPH444177:GJEL-232-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_LYSSC
AccessionPrimary (citable) accession number: B1HTV8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways