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B1HRT3 (BIOB_LYSSC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Bsph_3466
OrganismLysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP]
Taxonomic identifier444177 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Biotin synthase HAMAP-Rule MF_01694
PRO_0000381449

Sites

Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1081Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1401Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2001Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2701Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1HRT3 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: CFCC13B10F9C2D59

FASTA33136,788
        10         20         30         40         50         60 
MNFLQVAQEV IDGKIISNEE ALAILNSKDD ELLQLMDGAF AIRRHYYGKK VKLNMIMNAK 

        70         80         90        100        110        120 
SGYCPEDCGY CSQSSKSTAP IEKYPFITKE EILAGAKRAF DNKIGTYCIV ASGRGPTRKD 

       130        140        150        160        170        180 
VNVVSEAVVE IKEKYGLKVC ACLGLLKEEQ AQQLKEAGVD RYNHNLNTSE RHHSFITTSH 

       190        200        210        220        230        240 
TYEDRVNTVE IVKKHGISPC SGAIIGMKET KEDVVNIARA LHQLDADSIP VNFLNAIDGT 

       250        260        270        280        290        300 
KLEGTKDLNP RYCLKVLALF RYINPTKEIR ISGGREINLG CLQPLGLYAA NSIFVGDYLT 

       310        320        330 
TAGQEANSDY RMLEDLGFEI ELTQKQEAAF C 

« Hide

References

[1]"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C3-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000817 Genomic DNA. Translation: ACA40956.1.
RefSeqYP_001699086.1. NC_010382.1.

3D structure databases

ProteinModelPortalB1HRT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING444177.Bsph_3466.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA40956; ACA40956; Bsph_3466.
GeneID6022086.
KEGGlsp:Bsph_3466.
PATRIC22419373. VBILysSph89750_3544.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycLSPH444177:GJEL-3450-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_LYSSC
AccessionPrimary (citable) accession number: B1HRT3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways