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B1HRT3

- BIOB_LYSSC

UniProt

B1HRT3 - BIOB_LYSSC

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Protein

Biotin synthase

Gene
bioB, Bsph_3466
Organism
Lysinibacillus sphaericus (strain C3-41)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi71 – 711Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi108 – 1081Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi140 – 1401Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi200 – 2001Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi270 – 2701Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-3450-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Bsph_3466
OrganismiLysinibacillus sphaericus (strain C3-41)
Taxonomic identifieri444177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus
ProteomesiUP000002164: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Biotin synthaseUniRule annotationPRO_0000381449Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi444177.Bsph_3466.

Structurei

3D structure databases

ProteinModelPortaliB1HRT3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B1HRT3-1 [UniParc]FASTAAdd to Basket

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MNFLQVAQEV IDGKIISNEE ALAILNSKDD ELLQLMDGAF AIRRHYYGKK    50
VKLNMIMNAK SGYCPEDCGY CSQSSKSTAP IEKYPFITKE EILAGAKRAF 100
DNKIGTYCIV ASGRGPTRKD VNVVSEAVVE IKEKYGLKVC ACLGLLKEEQ 150
AQQLKEAGVD RYNHNLNTSE RHHSFITTSH TYEDRVNTVE IVKKHGISPC 200
SGAIIGMKET KEDVVNIARA LHQLDADSIP VNFLNAIDGT KLEGTKDLNP 250
RYCLKVLALF RYINPTKEIR ISGGREINLG CLQPLGLYAA NSIFVGDYLT 300
TAGQEANSDY RMLEDLGFEI ELTQKQEAAF C 331
Length:331
Mass (Da):36,788
Last modified:April 29, 2008 - v1
Checksum:iCFCC13B10F9C2D59
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000817 Genomic DNA. Translation: ACA40956.1.
RefSeqiYP_001699086.1. NC_010382.1.

Genome annotation databases

EnsemblBacteriaiACA40956; ACA40956; Bsph_3466.
GeneIDi6022086.
KEGGilsp:Bsph_3466.
PATRICi22419373. VBILysSph89750_3544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000817 Genomic DNA. Translation: ACA40956.1 .
RefSeqi YP_001699086.1. NC_010382.1.

3D structure databases

ProteinModelPortali B1HRT3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 444177.Bsph_3466.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACA40956 ; ACA40956 ; Bsph_3466 .
GeneIDi 6022086.
KEGGi lsp:Bsph_3466.
PATRICi 22419373. VBILysSph89750_3544.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci LSPH444177:GJEL-3450-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
    Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
    J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C3-41.

Entry informationi

Entry nameiBIOB_LYSSC
AccessioniPrimary (citable) accession number: B1HRT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: April 29, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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