B1HRL0 (B1HRL0_LYSSC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
S-ribosylhomocysteine lyase HAMAP MF_00091
EC=4.4.1.21 HAMAP MF_00091

Alternative name(s):
AI-2 synthesis protein HAMAP MF_00091
Autoinducer-2 production protein LuxS HAMAP MF_00091

Gene names

Name:	luxS HAMAP MF_00091
Ordered Locus Names:	Bsph_1699

Organism

Lysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP] EMBL ACA39295.1

Taxonomic identifier

444177 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Lysinibacillus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5-dihydroxy-2,3-pentadione (DPD) By similarity. HAMAP MF_00091 SAAS SAAS003815
Catalytic activity	S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione. HAMAP MF_00091 SAAS SAAS003815
Cofactor	Binds 1 iron ion per subunit By similarity. HAMAP MF_00091 SAAS SAAS003815
Subunit structure	Homodimer By similarity. HAMAP MF_00091 SAAS SAAS003815
Sequence similarities	Belongs to the LuxS family. HAMAP MF_00091

Ontologies

Keywords
Biological process	Autoinducer synthesis HAMAP MF_00091 SAAS SAAS003815 Quorum sensing HAMAP MF_00091 SAAS SAAS003815
Ligand	Iron HAMAP MF_00091 SAAS SAAS003815 Metal-binding HAMAP MF_00091 SAAS SAAS003815
Molecular function	Lyase HAMAP MF_00091 SAAS SAAS003815 EMBL ACA39295.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	quorum sensing Inferred from electronic annotation. Source: HAMAP
Molecular function	S-ribosylhomocysteine lyase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	58	1	Iron By similarity HAMAP MF_00091
	Metal binding	62	1	Iron By similarity HAMAP MF_00091
	Metal binding	125	1	Iron By similarity HAMAP MF_00091

Sequences

	Sequence		Length	Mass (Da)
	B1HRL0 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 71BA739FB47666A4	FASTA	156	17,747
	10 20 30 40 50 60 MQKEKTNVES FDLDHTKVKA PYVRLAGVKT GKNGDEVYKY DIRFKQPNKE HMEMPALHSL 70 80 90 100 110 120 EHLSADIIRN YSDHIVDFSP MGCQTGFYVS LINHNDYEDL LAILENTFTD VTKATAVPAC 130 140 150 NEVQCGWAAS HSLEGAKALA KEFLAKRDEW HIIFED

References

[1]

"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species." Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z. J. Bacteriol. 190:2892-2902(2008) [PubMed: 18296527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000817 Genomic DNA. Translation: ACA39295.1.
RefSeq	YP_001697425.1. NC_010382.1.
3D structure databases
ProteinModelPortal	B1HRL0.
SMR	B1HRL0. Positions 5-150.
ModBase	Search...
Protein-protein interaction databases
STRING	B1HRL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6023798.
GenomeReviews	Gene locus Bsph_1699 in contig CP000817_GR.
KEGG	lsp:Bsph_1699.
PATRIC	22415897. VBILysSph89750_1815.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG347473.
OMA	PHGDTIT.
ProtClustDB	PRK02260.
Family and domain databases
HAMAP	MF_00091. LuxS. [Tree]
InterPro	IPR011525. AuxIAA_ARF_dimer. IPR011249. Metalloenz_metal-bd. IPR003815. S-ribosylhomocysteinase. [Graphical view]
Gene3D	G3DSA:3.30.1360.80. S-ribosylhomocysteinase. 1 hit.
KO	K07173.
Pfam	PF02664. LuxS. 1 hit. [Graphical view]
PIRSF	PIRSF006160. AI2. 1 hit.
PRINTS	PR01487. LUXSPROTEIN.
ProDom	PD013172. S-ribosylhomocysteinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF63411. Metalloenz_metal-bd. 1 hit.
PROSITE	PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1HRL0_LYSSC

Accession

Primary (citable) accession number: B1HRL0

Entry history

Integrated into UniProtKB/TrEMBL:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)