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Protein

Translation initiation factor IF-2

Gene

infB

Organism
Lysinibacillus sphaericus (strain C3-41)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 275GTPUniRule annotation8
Nucleotide bindingi314 – 318GTPUniRule annotation5
Nucleotide bindingi368 – 371GTPUniRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-2UniRule annotation
Gene namesi
Name:infBUniRule annotation
Ordered Locus Names:Bsph_1598
OrganismiLysinibacillus sphaericus (strain C3-41)
Taxonomic identifieri444177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus
Proteomesi
  • UP000002164 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000938011 – 758Translation initiation factor IF-2Add BLAST758

Interactioni

Protein-protein interaction databases

STRINGi444177.Bsph_1598.

Structurei

3D structure databases

ProteinModelPortaliB1HR05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini259 – 428tr-type GAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 275G1By similarity8
Regioni293 – 297G2By similarity5
Regioni314 – 317G3By similarity4
Regioni368 – 371G4By similarity4
Regioni404 – 406G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QHU. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076906.
KOiK02519.
OMAiETEITHD.
OrthoDBiPOG091H00EY.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1HR05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKIRVHEYA KQVNKTSKEV IEALSKLNVS VTNHMSMLEK DIVSKLNQTF
60 70 80 90 100
KATPEKNVGK QATQNISQKS QSNGQQNHSV KKQEGQRQQS ATSKPKVNNQ
110 120 130 140 150
QHSNSSNEKS KNTKGNQNRN MTQNNNNNNN NNNNNRRGGG GYNQRPKPGI
160 170 180 190 200
HGGKRRHPKT HQPSIPVKQK ELPEKITFVE SLSVAELAKK LHREPSEIIK
210 220 230 240 250
KLFMLGVMAT INQELDKDAI ELICADYGVE VEEEIRVDIT DLETHFEQTE
260 270 280 290 300
EVNEAQLSER PPVVTIMGHV DHGKTTLLDS IRNTKVTAGE AGGITQHIGA
310 320 330 340 350
YQVTEGDKKI TFLDTPGHAA FTTMRARGAK VTDLTILVVA ADDGVMPQTV
360 370 380 390 400
EAINHAKAAE VPIIVAVNKM DKPSANPDRV MQELTEHGLV PEAWGGETIF
410 420 430 440 450
VPISALKGEG IDTLLEMILL VAEVGELKAN PDRLALGTVI EAQLDKGRGS
460 470 480 490 500
VATLLVQDGT LKVGDPIVVG HTFGRVRAMV NDKGRRVKEA GPSTPVEITG
510 520 530 540 550
LNDVPQAGDR FVVFEDEKTA RQVGETRAMS AIQAQRSEKQ RVTLDNLFEQ
560 570 580 590 600
MSQGEMKELN LIVKADVQGT VEAMAASLMK IDVEGVNVKI IHTGAGAITE
610 620 630 640 650
SDISLAAASN AIVIGFNVRP DVNAKRAAEE EGVDIRLHRI IYKVIEEIEQ
660 670 680 690 700
AMKGMLDPEF EEKIIGQAEV RQTIKVSKVG TIAGSYVTEG KVTRDSGVRV
710 720 730 740 750
IRDNVVIFEG ELDTLKRFKD EVKEVARGYE CGITITNFND IKEGDIIEAY

IMEEVKRV
Length:758
Mass (Da):83,264
Last modified:April 29, 2008 - v1
Checksum:i91E4CC40EF3546E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA39196.1.

Genome annotation databases

EnsemblBacteriaiACA39196; ACA39196; Bsph_1598.
KEGGilsp:Bsph_1598.
PATRICi22415693. VBILysSph89750_1713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA39196.1.

3D structure databases

ProteinModelPortaliB1HR05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444177.Bsph_1598.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA39196; ACA39196; Bsph_1598.
KEGGilsp:Bsph_1598.
PATRICi22415693. VBILysSph89750_1713.

Phylogenomic databases

eggNOGiENOG4107QHU. Bacteria.
COG0532. LUCA.
HOGENOMiHOG000076906.
KOiK02519.
OMAiETEITHD.
OrthoDBiPOG091H00EY.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00100_B. IF_2_B. 1 hit.
InterProiIPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
PS01176. IF2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2_LYSSC
AccessioniPrimary (citable) accession number: B1HR05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.