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B1HR05 (IF2_LYSSC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:Bsph_1598
OrganismLysinibacillus sphaericus (strain C3-41) [Complete proteome] [HAMAP]
Taxonomic identifier444177 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus

Protein attributes

Sequence length758 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 758758Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_1000093801

Regions

Nucleotide binding268 – 2758GTP By similarity
Nucleotide binding314 – 3185GTP By similarity
Nucleotide binding368 – 3714GTP By similarity
Region262 – 410149G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
B1HR05 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 91E4CC40EF3546E0

FASTA75883,264
        10         20         30         40         50         60 
MTKIRVHEYA KQVNKTSKEV IEALSKLNVS VTNHMSMLEK DIVSKLNQTF KATPEKNVGK 

        70         80         90        100        110        120 
QATQNISQKS QSNGQQNHSV KKQEGQRQQS ATSKPKVNNQ QHSNSSNEKS KNTKGNQNRN 

       130        140        150        160        170        180 
MTQNNNNNNN NNNNNRRGGG GYNQRPKPGI HGGKRRHPKT HQPSIPVKQK ELPEKITFVE 

       190        200        210        220        230        240 
SLSVAELAKK LHREPSEIIK KLFMLGVMAT INQELDKDAI ELICADYGVE VEEEIRVDIT 

       250        260        270        280        290        300 
DLETHFEQTE EVNEAQLSER PPVVTIMGHV DHGKTTLLDS IRNTKVTAGE AGGITQHIGA 

       310        320        330        340        350        360 
YQVTEGDKKI TFLDTPGHAA FTTMRARGAK VTDLTILVVA ADDGVMPQTV EAINHAKAAE 

       370        380        390        400        410        420 
VPIIVAVNKM DKPSANPDRV MQELTEHGLV PEAWGGETIF VPISALKGEG IDTLLEMILL 

       430        440        450        460        470        480 
VAEVGELKAN PDRLALGTVI EAQLDKGRGS VATLLVQDGT LKVGDPIVVG HTFGRVRAMV 

       490        500        510        520        530        540 
NDKGRRVKEA GPSTPVEITG LNDVPQAGDR FVVFEDEKTA RQVGETRAMS AIQAQRSEKQ 

       550        560        570        580        590        600 
RVTLDNLFEQ MSQGEMKELN LIVKADVQGT VEAMAASLMK IDVEGVNVKI IHTGAGAITE 

       610        620        630        640        650        660 
SDISLAAASN AIVIGFNVRP DVNAKRAAEE EGVDIRLHRI IYKVIEEIEQ AMKGMLDPEF 

       670        680        690        700        710        720 
EEKIIGQAEV RQTIKVSKVG TIAGSYVTEG KVTRDSGVRV IRDNVVIFEG ELDTLKRFKD 

       730        740        750 
EVKEVARGYE CGITITNFND IKEGDIIEAY IMEEVKRV 

« Hide

References

[1]"Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C3-41.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000817 Genomic DNA. Translation: ACA39196.1.
RefSeqYP_001697326.1. NC_010382.1.

3D structure databases

ProteinModelPortalB1HR05.
SMRB1HR05. Positions 532-651, 660-757.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING444177.Bsph_1598.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA39196; ACA39196; Bsph_1598.
GeneID6021487.
KEGGlsp:Bsph_1598.
PATRIC22415693. VBILysSph89750_1713.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076906.
KOK02519.
OMAMKGQLEP.
OrthoDBEOG67HJSV.
ProtClustDBPRK05306.

Enzyme and pathway databases

BioCycLSPH444177:GJEL-1639-MONOMER.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF04760. IF2_N. 2 hits.
[Graphical view]
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_LYSSC
AccessionPrimary (citable) accession number: B1HR05
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries