ID   B1HPE2_LYSSC            Unreviewed;       605 AA.
AC   B1HPE2;
DT   29-APR-2008, integrated into UniProtKB/TrEMBL.
DT   29-APR-2008, sequence version 1.
DT   27-MAR-2024, entry version 80.
DE   RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE            EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE   AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN   OrderedLocusNames=Bsph_3075 {ECO:0000313|EMBL:ACA40588.1};
OS   Lysinibacillus sphaericus (strain C3-41).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX   NCBI_TaxID=444177 {ECO:0000313|EMBL:ACA40588.1, ECO:0000313|Proteomes:UP000002164};
RN   [1] {ECO:0000313|EMBL:ACA40588.1, ECO:0000313|Proteomes:UP000002164}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C3-41 {ECO:0000313|EMBL:ACA40588.1,
RC   ECO:0000313|Proteomes:UP000002164};
RX   PubMed=18296527; DOI=10.1128/JB.01652-07;
RA   Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M.,
RA   Berry C., Yuan Z.;
RT   "Complete genome sequence of the mosquitocidal bacterium Bacillus
RT   sphaericus C3-41 and comparison with those of closely related Bacillus
RT   species.";
RL   J. Bacteriol. 190:2892-2902(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC         diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
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DR   EMBL; CP000817; ACA40588.1; -; Genomic_DNA.
DR   RefSeq; WP_012294672.1; NC_010382.1.
DR   AlphaFoldDB; B1HPE2; -.
DR   EnsemblBacteria; ACA40588; ACA40588; Bsph_3075.
DR   KEGG; lsp:Bsph_3075; -.
DR   HOGENOM; CLU_008325_0_2_9; -.
DR   Proteomes; UP000002164; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR   CDD; cd04866; LigD_Pol_like_3; 1.
DR   Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR   Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR014146; LigD_ligase_dom.
DR   InterPro; IPR033652; LigD_Pol-like_3.
DR   InterPro; IPR014145; LigD_pol_dom.
DR   InterPro; IPR014143; NHEJ_ligase_prk.
DR   NCBIfam; TIGR02778; ligD_pol; 1.
DR   NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR   NCBIfam; TIGR02776; NHEJ_ligase_prk; 1.
DR   PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR   PANTHER; PTHR42705:SF2; MULTIFUNCTIONAL NON-HOMOLOGOUS END JOINING DNA REPAIR PROTEIN LIGD; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF21686; LigD_Prim-Pol; 1.
DR   SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW   DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW   DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW   Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:ACA40588.1};
KW   Manganese {ECO:0000256|ARBA:ARBA00023211};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          15..205
FT                   /note="ATP-dependent DNA ligase family profile"
FT                   /evidence="ECO:0000259|Pfam:PF01068"
FT   DOMAIN          336..584
FT                   /note="DNA ligase D polymerase"
FT                   /evidence="ECO:0000259|Pfam:PF21686"
SQ   SEQUENCE   605 AA;  70532 MW;  5FFA5C63F6450C11 CRC64;
     MKPMLLTEAN EIPIGDDWLY ETKYDGFRCL LIWDEQPILI SRNGRNLNHS FPEIIDYCHQ
     IHASVKAFLP LIFDGELVYL SNPYKSEFSV VQQRGRMQNQ DVIAKHAQAF PMRLLVFDML
     MIKGESFSNR YLKTRKEQLA KLAAKVKLPN VDDQDGKVIQ IVQAKEESNI LWQAIKVYNG
     EGMVAKKKTS KWLDNTRSSH WLKIKNWRYV TVIVTQYDHS NGYFQGSIYD GGNLREVVTF
     KHGMKEEEHQ TLVAFFQAQG QRKKELWELE PSICVDIACI DFDGSKLREP KFHAFRLELT
     PDICQWQHMQ RQLFPIPETV PITHPDKPVW PEIGVTKDHY LSYLQSVSPY LLPFLQDRPL
     TLIRYPHGVP GESFYQKSRP DKLPDFVGTA MMDEIDYMVC NNLETLLWLG NQLALEWHIP
     FQTRQTMNPT EIVFDLDPPS TDHFSLAIAG ALDLKGIIDY FQLQSFVKTS GGKGLQLYIP
     LPANKFTYEE VRVFTEFACR FLCQQKPHLY TIERLKKNRH NKLYLDYVQH AEGKTIIAPY
     STRGNELGLV ATPLHWEEVN DDLKPYYFSI PAVMERMKKI SNPFKYFREV GEQQDFQAVL
     DRINE
//