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Protein

NAD kinase

Gene

nadK

Organism
Lysinibacillus sphaericus (strain C3-41)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Proton acceptorUniRule annotation
Binding sitei50 – 501NADUniRule annotation
Binding sitei147 – 1471NADUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei184 – 1841NAD; via carbonyl oxygenUniRule annotation
Binding sitei224 – 2241NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 462NADUniRule annotation
Nucleotide bindingi121 – 1222NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-1273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:Bsph_1227
OrganismiLysinibacillus sphaericus (strain C3-41)
Taxonomic identifieri444177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus
ProteomesiUP000002164: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264NAD kinasePRO_1000120872Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi444177.Bsph_1227.

Structurei

3D structure databases

ProteinModelPortaliB1HNY3.
SMRiB1HNY3. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiIQMSEIA.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B1HNY3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKFAIQSRRD AQSNELMELA KTYLQDFGLT YDEEAPEIVV SIGGDGTLLH
60 70 80 90 100
AFHRYSHLLD QVAFVGIHTG HLGFYADWKP SELEKLVLSI AKKDFNVVEY
110 120 130 140 150
PLLEVKVEHH NAASNTYLAL NEATVKSPDV TLVMDVELNG NQFERNRGDG
160 170 180 190 200
HSVSTPSGST AYNKALGGAI IHPTLAALQI TEIASINNRV FRTVGSPLIL
210 220 230 240 250
PAHHHCVLRP VNEQNFNMTV DHLQITQGDV KAIVFNVANE RVRFARFRPF
260
PFWERVHESF VANE
Length:264
Mass (Da):29,586
Last modified:April 29, 2008 - v1
Checksum:iFD12FA976C172ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA38835.1.
RefSeqiYP_001696965.1. NC_010382.1.

Genome annotation databases

EnsemblBacteriaiACA38835; ACA38835; Bsph_1227.
GeneIDi6022237.
KEGGilsp:Bsph_1227.
PATRICi22414948. VBILysSph89750_1344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA38835.1.
RefSeqiYP_001696965.1. NC_010382.1.

3D structure databases

ProteinModelPortaliB1HNY3.
SMRiB1HNY3. Positions 1-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444177.Bsph_1227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA38835; ACA38835; Bsph_1227.
GeneIDi6022237.
KEGGilsp:Bsph_1227.
PATRICi22414948. VBILysSph89750_1344.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiIQMSEIA.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-1273-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
    Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
    J. Bacteriol. 190:2892-2902(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C3-41.

Entry informationi

Entry nameiNADK_LYSSC
AccessioniPrimary (citable) accession number: B1HNY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: February 4, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.