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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Lysinibacillus sphaericus (strain C3-41)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081ZincUniRule annotation
Metal bindingi110 – 1101ZincUniRule annotation
Metal bindingi136 – 1361ZincUniRule annotation
Metal bindingi138 – 1381ZincUniRule annotation
Binding sitei256 – 2561ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-4643-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Bsph_4644
OrganismiLysinibacillus sphaericus (strain C3-41)
Taxonomic identifieri444177 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeLysinibacillus
ProteomesiUP000002164 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Glutamate--tRNA ligasePRO_1000090086Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi444177.Bsph_4644.

Structurei

3D structure databases

ProteinModelPortaliB1HNM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi253 – 2575"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1HNM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKEVRVRYA PSPTGFLHIG GARTALFNYL YAKHHNGKFI VRIEDTDIER
60 70 80 90 100
NVEGGEASQL DNLKWLGIEY DESIDIGGPY APYRQMERLD IYKEHAEKLL
110 120 130 140 150
AQGAAYKCFC SSEKLEASRE EQKARGVAAP TYDGTCRHLS AEEVAAKEAA
160 170 180 190 200
GEPYTIRMRV PENVTYDFED LVRGQVTFES KDIGDWVIVK ANGIPTYNYA
210 220 230 240 250
VVLDDHFMEI SHVFRGEEHL SNTPKQMMIF DAFGWEYPRF GHMTLIINEN
260 270 280 290 300
RKKLSKRDES IIQFVTQYKD LGYLPEAMFN FFALLGWSPE GEEEIFSKEE
310 320 330 340 350
FIKIFDEKRL SKSPSMFDKQ KLTWMNNQYI KKLSLEEVVA LSLPHLQKAG
360 370 380 390 400
LLPDELSAVQ HAWATDLIGL YHDQMSFGAE IVELSSLFFK DHIEYDEEAK
410 420 430 440 450
AVLAGEQVPE VMAAFKAQLE ELEEFTPDAV KAAIKAVQKA TGHKGKNLFM
460 470 480
PIRVVTTGET HGPELPNAIC LIGKEKAIDR VEKFAQ
Length:486
Mass (Da):55,274
Last modified:April 28, 2008 - v1
Checksum:i7CDABB981E3A5CB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA42088.1.
RefSeqiYP_001700218.1. NC_010382.1.

Genome annotation databases

EnsemblBacteriaiACA42088; ACA42088; Bsph_4644.
KEGGilsp:Bsph_4644.
PATRICi22421807. VBILysSph89750_4721.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000817 Genomic DNA. Translation: ACA42088.1.
RefSeqiYP_001700218.1. NC_010382.1.

3D structure databases

ProteinModelPortaliB1HNM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444177.Bsph_4644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACA42088; ACA42088; Bsph_4644.
KEGGilsp:Bsph_4644.
PATRICi22421807. VBILysSph89750_4721.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK09698.
OMAiKLNWINS.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciLSPH444177:GJEL-4643-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of the mosquitocidal bacterium Bacillus sphaericus C3-41 and comparison with those of closely related Bacillus species."
    Hu X., Fan W., Han B., Liu H., Zheng D., Li Q., Dong W., Yan J., Gao M., Berry C., Yuan Z.
    J. Bacteriol. 190:2892-2902(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C3-41.

Entry informationi

Entry nameiSYE_LYSSC
AccessioniPrimary (citable) accession number: B1HNM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: April 28, 2008
Last modified: March 31, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.