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B1H3E1

- NLK2_XENTR

UniProt

B1H3E1 - NLK2_XENTR

Protein

Serine/threonine-protein kinase NLK2

Gene

nlk.2

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (29 Apr 2008)
      Previous versions | rss
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    Functioni

    Negatively regulates Wnt/beta-catenin-signaling during development. Plays a role together with sox11 in neural induction during early embryogenesis. Involved in TGFbeta-mediated mesoderm induction in early embryos, acting downstream of map3k7/tak1 to phosphorylate stat3. Augments the rnf138/narf-directed ubiquitination and degradation of tcf/lef by enhancing the association of rnf138/narf and tcf/lef. Phosphorylates mef2a to play a role in anterior neural development, including eye formation By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961ATPBy similarityPROSITE-ProRule annotation
    Active sitei193 – 1931Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 819ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. magnesium ion binding Source: UniProtKB
    3. MAP kinase activity Source: UniProtKB-EC
    4. protein serine/threonine kinase activity Source: UniProtKB
    5. transcription factor binding Source: UniProtKB
    6. ubiquitin protein ligase binding Source: UniProtKB

    GO - Biological processi

    1. anterior/posterior pattern specification Source: UniProtKB
    2. mesoderm formation Source: UniProtKB
    3. nervous system development Source: UniProtKB
    4. peptidyl-serine phosphorylation Source: UniProtKB
    5. peptidyl-threonine phosphorylation Source: UniProtKB
    6. positive regulation of protein ubiquitination Source: UniProtKB
    7. regulation of transcription, DNA-templated Source: UniProtKB-KW
    8. serine phosphorylation of STAT3 protein Source: UniProtKB
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. transforming growth factor beta receptor signaling pathway Source: UniProtKB
    11. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation, Ubl conjugation pathway, Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase NLK2 (EC:2.7.11.24)
    Alternative name(s):
    Nemo-like kinase 2
    Short name:
    Nlk.2
    Gene namesi
    Name:nlk.2
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-1218923. nlk.2.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 454454Serine/threonine-protein kinase NLK2PRO_0000370236Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with sox11, hmgxb4/hmg2l1, rnf138/narf, stat3.1 and mef2a.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliB1H3E1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini67 – 356290Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi36 – 427Poly-GlySequence Analysis

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    KOiK04468.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1H3E1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFQGPGRSL PGQLCAGVFG GLIQPPLGQK FYCPNGGSGG GGVPAVPSPL    50
    PQALSAPQCN GDGRGEPEPD RPIGYGAFGV VWSVTDPRDG KRVALKKMPN 100
    VFQNLVSCKR VFRELKMLCF FKHDNVLSAL DILQPPQIDC FEEIYVITEL 150
    MQTDLHKVIV SPQPLSSDHI KVFLYQILRG LKYLHSAGIL HRDIKPGNLL 200
    VNSNCVLKIC DFGLARVEEL DESQHMTQEV VTQYYRAPEI LMGSRHYRSA 250
    IDIWSVGCIF AELLGRRILF QAQSPIQQLD LITDLLGTPP LTAMRSACEG 300
    ARAHILRGPH KPPSLSVLYM LSGEATHEAV HLLCRMLLFD PLKRISAKDA 350
    LAHPYLEEGR LRYHTCMCHC CYSVSSGRVY TADFEPTATN RFDDSYEKSL 400
    TSVWQVKELV HRFITDQQQG KRPPLCINPH SAAFKTFIRS TAWHSSKVSK 450
    KEER 454
    Length:454
    Mass (Da):50,652
    Last modified:April 29, 2008 - v1
    Checksum:i8DD61C2D6CCEEC4F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161361 mRNA. Translation: AAI61361.1.
    RefSeqiNP_001116917.1. NM_001123445.1.
    UniGeneiStr.64836.

    Genome annotation databases

    GeneIDi100144684.
    KEGGixtr:100144684.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC161361 mRNA. Translation: AAI61361.1 .
    RefSeqi NP_001116917.1. NM_001123445.1.
    UniGenei Str.64836.

    3D structure databases

    ProteinModelPortali B1H3E1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100144684.
    KEGGi xtr:100144684.

    Organism-specific databases

    CTDi 398295.
    Xenbasei XB-GENE-1218923. nlk.2.

    Phylogenomic databases

    eggNOGi COG0515.
    KOi K04468.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Xenopus Gene Collection (XGC) project
      Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: NeurulaImported.

    Entry informationi

    Entry nameiNLK2_XENTR
    AccessioniPrimary (citable) accession number: B1H3E1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3