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Protein

Serine/threonine-protein kinase NLK2

Gene

nlk.2

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Negatively regulates Wnt/beta-catenin-signaling during development. Plays a role together with sox11 in neural induction during early embryogenesis. Involved in TGFbeta-mediated mesoderm induction in early embryos, acting downstream of map3k7/tak1 to phosphorylate stat3. Augments the rnf138/narf-directed ubiquitination and degradation of tcf/lef by enhancing the association of rnf138/narf and tcf/lef. Phosphorylates mef2a to play a role in anterior neural development, including eye formation (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961ATPPROSITE-ProRule annotationBy similarity
Active sitei193 – 1931Proton acceptorPROSITE-ProRule annotationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 819ATPPROSITE-ProRule annotationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase NLK2 (EC:2.7.11.24)
Alternative name(s):
Nemo-like kinase 2
Short name:
Nlk.2
Gene namesi
Name:nlk.2
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-1218923. nlk.2.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 454454Serine/threonine-protein kinase NLK2PRO_0000370236Add
BLAST

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with sox11, hmgxb4/hmg2l1, rnf138/narf, stat3.1 and mef2a.By similarity

Protein-protein interaction databases

STRINGi8364.ENSXETP00000024801.

Structurei

3D structure databases

ProteinModelPortaliB1H3E1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 356290Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi36 – 427Poly-GlySequence Analysis

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiB1H3E1.
KOiK04468.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1H3E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFQGPGRSL PGQLCAGVFG GLIQPPLGQK FYCPNGGSGG GGVPAVPSPL
60 70 80 90 100
PQALSAPQCN GDGRGEPEPD RPIGYGAFGV VWSVTDPRDG KRVALKKMPN
110 120 130 140 150
VFQNLVSCKR VFRELKMLCF FKHDNVLSAL DILQPPQIDC FEEIYVITEL
160 170 180 190 200
MQTDLHKVIV SPQPLSSDHI KVFLYQILRG LKYLHSAGIL HRDIKPGNLL
210 220 230 240 250
VNSNCVLKIC DFGLARVEEL DESQHMTQEV VTQYYRAPEI LMGSRHYRSA
260 270 280 290 300
IDIWSVGCIF AELLGRRILF QAQSPIQQLD LITDLLGTPP LTAMRSACEG
310 320 330 340 350
ARAHILRGPH KPPSLSVLYM LSGEATHEAV HLLCRMLLFD PLKRISAKDA
360 370 380 390 400
LAHPYLEEGR LRYHTCMCHC CYSVSSGRVY TADFEPTATN RFDDSYEKSL
410 420 430 440 450
TSVWQVKELV HRFITDQQQG KRPPLCINPH SAAFKTFIRS TAWHSSKVSK

KEER
Length:454
Mass (Da):50,652
Last modified:April 29, 2008 - v1
Checksum:i8DD61C2D6CCEEC4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161361 mRNA. Translation: AAI61361.1.
RefSeqiNP_001116917.1. NM_001123445.1.
UniGeneiStr.64836.

Genome annotation databases

GeneIDi100144684.
KEGGixtr:100144684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161361 mRNA. Translation: AAI61361.1.
RefSeqiNP_001116917.1. NM_001123445.1.
UniGeneiStr.64836.

3D structure databases

ProteinModelPortaliB1H3E1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000024801.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100144684.
KEGGixtr:100144684.

Organism-specific databases

CTDi398295.
XenbaseiXB-GENE-1218923. nlk.2.

Phylogenomic databases

eggNOGiCOG0515.
InParanoidiB1H3E1.
KOiK04468.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: NeurulaImported.

Entry informationi

Entry nameiNLK2_XENTR
AccessioniPrimary (citable) accession number: B1H3E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: June 24, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.