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B1H3E1 (NLK2_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase NLK2

EC=2.7.11.24
Alternative name(s):
Nemo-like kinase 2
Short name=Nlk.2
Gene names
Name:nlk.2
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length454 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Negatively regulates Wnt/beta-catenin-signaling during development. Plays a role together with sox11 in neural induction during early embryogenesis. Involved in TGFbeta-mediated mesoderm induction in early embryos, acting downstream of map3k7/tak1 to phosphorylate stat3. Augments the rnf138/narf-directed ubiquitination and degradation of tcf/lef by enhancing the association of rnf138/narf and tcf/lef. Phosphorylates mef2a to play a role in anterior neural development, including eye formation By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q8QGV6

Cofactor

Magnesium By similarity. UniProtKB O54949

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity. UniProtKB Q63844

Subunit structure

Interacts with sox11, hmgxb4/hmg2l1, rnf138/narf, stat3.1 and mef2a By similarity. UniProtKB Q8QGV6

Subcellular location

Nucleus By similarity. Cytoplasm By similarity UniProtKB O54949.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
Ubl conjugation pathway
Wnt signaling pathway
   Cellular componentCytoplasm
Nucleus
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

anterior/posterior pattern specification

Inferred from sequence or structural similarity. Source: UniProtKB

mesoderm formation

Inferred from sequence or structural similarity. Source: UniProtKB

nervous system development

Inferred from sequence or structural similarity. Source: UniProtKB

peptidyl-serine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

peptidyl-threonine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein ubiquitination

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

serine phosphorylation of STAT3 protein

Inferred from sequence or structural similarity. Source: UniProtKB

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transforming growth factor beta receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from sequence or structural similarity. Source: UniProtKB

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

magnesium ion binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein serine/threonine kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription factor binding

Inferred from sequence or structural similarity. Source: UniProtKB

ubiquitin protein ligase binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 454454Serine/threonine-protein kinase NLK2
PRO_0000370236

Regions

Domain67 – 356290Protein kinase
Nucleotide binding73 – 819ATP By similarity UniProtKB P53779
Compositional bias36 – 427Poly-Gly

Sites

Active site1931Proton acceptor By similarity UniProtKB P53779
Binding site961ATP By similarity UniProtKB P53779

Sequences

Sequence LengthMass (Da)Tools
B1H3E1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 8DD61C2D6CCEEC4F

FASTA45450,652
        10         20         30         40         50         60 
MAFQGPGRSL PGQLCAGVFG GLIQPPLGQK FYCPNGGSGG GGVPAVPSPL PQALSAPQCN 

        70         80         90        100        110        120 
GDGRGEPEPD RPIGYGAFGV VWSVTDPRDG KRVALKKMPN VFQNLVSCKR VFRELKMLCF 

       130        140        150        160        170        180 
FKHDNVLSAL DILQPPQIDC FEEIYVITEL MQTDLHKVIV SPQPLSSDHI KVFLYQILRG 

       190        200        210        220        230        240 
LKYLHSAGIL HRDIKPGNLL VNSNCVLKIC DFGLARVEEL DESQHMTQEV VTQYYRAPEI 

       250        260        270        280        290        300 
LMGSRHYRSA IDIWSVGCIF AELLGRRILF QAQSPIQQLD LITDLLGTPP LTAMRSACEG 

       310        320        330        340        350        360 
ARAHILRGPH KPPSLSVLYM LSGEATHEAV HLLCRMLLFD PLKRISAKDA LAHPYLEEGR 

       370        380        390        400        410        420 
LRYHTCMCHC CYSVSSGRVY TADFEPTATN RFDDSYEKSL TSVWQVKELV HRFITDQQQG 

       430        440        450 
KRPPLCINPH SAAFKTFIRS TAWHSSKVSK KEER 

« Hide

References

[1]NIH - Xenopus Gene Collection (XGC) project
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Neurula.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC161361 mRNA. Translation: AAI61361.1.
RefSeqNP_001116917.1. NM_001123445.1.
UniGeneStr.64836.

3D structure databases

ProteinModelPortalB1H3E1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100144684.
KEGGxtr:100144684.

Organism-specific databases

CTD398295.
XenbaseXB-GENE-1218923. nlk.2.

Phylogenomic databases

eggNOGCOG0515.
KOK04468.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNLK2_XENTR
AccessionPrimary (citable) accession number: B1H3E1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families