Reviewed, UniProtKB/Swiss-Prot B1H3C9 (OST48_XENTR)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119

Gene names

Name:

ddost

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 437	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000357450
Regions
	Topological domain	25 – 407	383	Lumenal Potential
	Transmembrane	408 – 428	21	Potential
	Topological domain	429 – 437	9	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	B1H3C9-1 [UniParc]. Last modified April 29, 2008. Version 1. Checksum: 7EFC5A3A302885B4	FASTA	437	48,265
	10 20 30 40 50 60 MASLRVSVLL VAASCLLLGS GLRAGPRTLV LLENINLRET HSLFFRSLSD RGFDLSFRTA 70 80 90 100 110 120 DDPSLSLIKY GEFLYDNLII FSPSVEDFGG NINIETISSF IDGGGSVLVA ASSDIGDPLR 130 140 150 160 170 180 ELGSECGIEF DEDKTAVIDH HNYDISDPGQ HSLIVADSES LLKAPTIVGK APLNPILFRG 190 200 210 220 230 240 VGMVADPDNP LVLDILTGSS TSYSFFPDKP ITQYPHAVGK NTLLIAGLQA RNNARVVFSG 250 260 270 280 290 300 SMDFFSDAFF NSAVQKAAGG SNRYAKTGNY ELAVALSRWV FKEEGVLRVG QVSHHRVGES 310 320 330 340 350 360 SPPSAYTVTD LVEYSIVIEQ LSNGKWVPFD GDDIQLEFVR IDPFVRTFLK KNGGKYSVQF 370 380 390 400 410 420 KLPDVYGVFQ FKVDYNRLGY THLYSTTQVS VRPLQHTQYE RFIPSAYPYY ASAFSVMFGL 430 FIFSIVFLHM KEKEKSD

References

[1]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC161349 mRNA. Translation: AAI61349.1.
RefSeq	NP_001120481.1.
UniGene	Str.25360
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B1H3C9.
Genome annotation databases
GeneID	100145597.
KEGG	xtr:100145597.
Organism-specific databases
CTD	100145597.
Xenbase	XB-FEAT-5784935. ddost.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_XENTR

Accession

Primary (citable) accession number: B1H3C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	April 29, 2008
Last modified:	October 13, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project