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Protein

Histone deacetylase 8

Gene

hdac8

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity).By similarity

Catalytic activityi

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone.

Cofactori

a divalent metal cationNote: Binds 1 divalent metal cation per subunit.

Enzyme regulationi

Its activity is inhibited by trichostatin A (TSA) and butyrate, 2 well known histone deacetylase inhibitors.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateBy similarity
Active sitei135 – 1351Proton acceptorBy similarity
Binding sitei143 – 1431Substrate; via carbonyl oxygenBy similarity
Metal bindingi170 – 1701Divalent metal cationBy similarity
Metal bindingi172 – 1721Divalent metal cationBy similarity
Metal bindingi259 – 2591Divalent metal cationBy similarity
Binding sitei298 – 2981SubstrateBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. NAD-dependent histone deacetylase activity (H3-K14 specific) Source: UniProtKB-EC
  3. NAD-dependent histone deacetylase activity (H3-K18 specific) Source: UniProtKB-EC
  4. NAD-dependent histone deacetylase activity (H3-K9 specific) Source: UniProtKB-EC
  5. NAD-dependent histone deacetylase activity (H4-K16 specific) Source: UniProtKB-EC

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Hydrolase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

ReactomeiREACT_276199. Separation of Sister Chromatids.
REACT_343839. HDACs deacetylate histones.
REACT_349176. Resolution of Sister Chromatid Cohesion.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone deacetylase 8 (EC:3.5.1.98)
Short name:
HD8
Gene namesi
Name:hdac8
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unplaced

Organism-specific databases

XenbaseiXB-GENE-5863426. hdac8.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Histone deacetylase 8PRO_0000389511Add
BLAST

Expressioni

Gene expression databases

BgeeiB1H369.
ExpressionAtlasiB1H369. baseline.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000044353.

Structurei

3D structure databases

ProteinModelPortaliB1H369.
SMRiB1H369. Positions 5-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 316312Histone deacetylaseBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0123.
GeneTreeiENSGT00530000062889.
HOGENOMiHOG000225180.
HOVERGENiHBG057112.
InParanoidiB1H369.
KOiK11405.
OMAiCLIDGKC.
OrthoDBiEOG722J95.
TreeFamiTF106175.

Family and domain databases

Gene3Di3.40.800.20. 1 hit.
InterProiIPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view]
PANTHERiPTHR10625. PTHR10625. 1 hit.
PfamiPF00850. Hist_deacetyl. 1 hit.
[Graphical view]
PIRSFiPIRSF037913. His_deacetylse_1. 1 hit.
PRINTSiPR01270. HDASUPER.
PR01271. HISDACETLASE.

Sequencei

Sequence statusi: Complete.

B1H369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEESLLPVYI HSAEYVELCD NVQSKVPRRA SMVHSLIEAY GLLKEMRVVK
60 70 80 90 100
PKVASMEEMA AFHTDSYLQH LHKVSEEGDN DDPETLEYGL GYDCPITEGI
110 120 130 140 150
YDYAAAVGGA TLTAAEQLMA GKTRIAINWP GGWHHAKKDE ASGFCYLNDA
160 170 180 190 200
VLGILKLREK FDRVLYVDMD LHHGDGVEDA FSFTSKVMTV SLHKFSPGFF
210 220 230 240 250
PGTGDVSDIG LGKGRYYSVN VPLQDGIQDE KYYQICEGVL KEVFTTFNPE
260 270 280 290 300
AVVLQLGADT IAGDPMCSFN MTPQGIGKCL KYVLQWQLPT LILGGGGYHL
310 320 330 340 350
PNTARCWTYL TALIVGRTLS SEIPDHEFFT EYGPDYVLEV TPSCRPDRND
360
SQKVQEILQS IKGHLKQVV
Length:369
Mass (Da):41,006
Last modified:April 28, 2008 - v1
Checksum:i9ED5BBE73D188A75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161282 mRNA. Translation: AAI61282.1.
RefSeqiNP_001120462.1. NM_001126990.1.
UniGeneiStr.52530.

Genome annotation databases

EnsembliENSXETT00000044353; ENSXETP00000044353; ENSXETG00000020527.
GeneIDi100145561.
KEGGixtr:100145561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC161282 mRNA. Translation: AAI61282.1.
RefSeqiNP_001120462.1. NM_001126990.1.
UniGeneiStr.52530.

3D structure databases

ProteinModelPortaliB1H369.
SMRiB1H369. Positions 5-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000044353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSXETT00000044353; ENSXETP00000044353; ENSXETG00000020527.
GeneIDi100145561.
KEGGixtr:100145561.

Organism-specific databases

CTDi55869.
XenbaseiXB-GENE-5863426. hdac8.

Phylogenomic databases

eggNOGiCOG0123.
GeneTreeiENSGT00530000062889.
HOGENOMiHOG000225180.
HOVERGENiHBG057112.
InParanoidiB1H369.
KOiK11405.
OMAiCLIDGKC.
OrthoDBiEOG722J95.
TreeFamiTF106175.

Enzyme and pathway databases

ReactomeiREACT_276199. Separation of Sister Chromatids.
REACT_343839. HDACs deacetylate histones.
REACT_349176. Resolution of Sister Chromatid Cohesion.

Gene expression databases

BgeeiB1H369.
ExpressionAtlasiB1H369. baseline.

Family and domain databases

Gene3Di3.40.800.20. 1 hit.
InterProiIPR000286. His_deacetylse.
IPR003084. His_deacetylse_1.
IPR023801. His_deacetylse_dom.
[Graphical view]
PANTHERiPTHR10625. PTHR10625. 1 hit.
PfamiPF00850. Hist_deacetyl. 1 hit.
[Graphical view]
PIRSFiPIRSF037913. His_deacetylse_1. 1 hit.
PRINTSiPR01270. HDASUPER.
PR01271. HISDACETLASE.
ProtoNetiSearch...

Publicationsi

  1. NIH - Xenopus Gene Collection (XGC) project
    Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.

Entry informationi

Entry nameiHDAC8_XENTR
AccessioniPrimary (citable) accession number: B1H369
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2009
Last sequence update: April 28, 2008
Last modified: March 31, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.